C57BL/6NCya-Atf3em1flox/Cya
Common Name:
Atf3-flox
Product ID:
S-CKO-01328
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Atf3-flox
Strain ID
CKOCMP-11910-Atf3-B6N-VA
Gene Name
Product ID
S-CKO-01328
Gene Alias
LRG-21
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Atf3em1flox/Cya mice (Catalog S-CKO-01328) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027941
NCBI RefSeq
NM_007498
Target Region
Exon 3~4
Size of Effective Region
~3.3 kb
Detailed Document
Overview of Gene Research
Atf3, or activating transcription factor 3, is a member of the ATF/CREB protein family. It acts as a transcription suppressor or activator, playing a vital role in the cellular adaptive-response network. Multiple extracellular signals, such as endoplasmic reticulum stress, cytokines, chemokines, and LPS, can induce its expression. Atf3 is involved in regulating various biological processes, including metabolism, immunity, and oncogenesis [4].
In abdominal aortic aneurysm (AAA), the deficiency of Atf3 in vascular smooth muscle cells promotes AAA formation in Ang II-induced AAA mice. PDGFRB was identified as its target, mediating vascular smooth muscle cell proliferation at the early stage of AAA, and Atf3 suppresses mitochondria-dependent apoptosis at the advanced stage by upregulating BCL2 [1].
In atherosclerosis, its role is controversial. In endothelial cells, overexpression aggravates oxidative stress and inflammation, while in macrophages and liver cells, it can act as a negative regulator of inflammation and promote cholesterol metabolism [2].
In metabolic dysfunction-associated steatohepatitis (MASH), Atf3 in macrophages regulates the glucose-fatty acid cycle, attenuating hepatocyte steatosis and fibrogenesis in hepatic stellate cells [3].
In conclusion, Atf3 is a key regulator in multiple biological processes and diseases. Gene knockout and conditional knockout mouse models have revealed its role in diseases like AAA, atherosclerosis, and MASH. Understanding Atf3's functions can potentially lead to new therapeutic strategies for these diseases.
References:
1. Wen, Ying, Liu, Yingying, Li, Qiang, Zhang, Yuan, Tang, Wai Ho. 2024. Spatiotemporal ATF3 Expression Determines VSMC Fate in Abdominal Aortic Aneurysm. In Circulation research, 134, 1495-1511. doi:10.1161/CIRCRESAHA.124.324323. https://pubmed.ncbi.nlm.nih.gov/38686580/
2. Wang, Bingyu, Yang, Xi, Sun, Xinyi, Lian, Jiangfang, Zhou, Jianqing. 2022. ATF3 in atherosclerosis: a controversial transcription factor. In Journal of molecular medicine (Berlin, Germany), 100, 1557-1568. doi:10.1007/s00109-022-02263-7. https://pubmed.ncbi.nlm.nih.gov/36207452/
3. Hu, Shuwei, Li, Rui, Gong, Dongxu, Wu, Huijuan, Xu, Yanyong. 2024. Atf3-mediated metabolic reprogramming in hepatic macrophage orchestrates metabolic dysfunction-associated steatohepatitis. In Science advances, 10, eado3141. doi:10.1126/sciadv.ado3141. https://pubmed.ncbi.nlm.nih.gov/39047111/
4. Ku, Hui-Chen, Cheng, Ching-Feng. 2020. Master Regulator Activating Transcription Factor 3 (ATF3) in Metabolic Homeostasis and Cancer. In Frontiers in endocrinology, 11, 556. doi:10.3389/fendo.2020.00556. https://pubmed.ncbi.nlm.nih.gov/32922364/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen