C57BL/6NCya-Bcl6em1flox/Cya
Common Name:
Bcl6-flox
Product ID:
S-CKO-01403
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Bcl6-flox
Strain ID
CKOCMP-12053-Bcl6-B6N-VA
Gene Name
Product ID
S-CKO-01403
Gene Alias
Bcl5
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Bcl6em1flox/Cya mice (Catalog S-CKO-01403) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000023151
NCBI RefSeq
NM_009744
Target Region
Exon 5~6
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
Bcl6, also known as B-cell lymphoma 6, is a transcriptional repressor that plays a crucial role in the development and maintenance of germinal centers (GCs), which are essential for an effective humoral immune response [2]. It is also involved in many other biological processes such as cell proliferation, survival, differentiation, migration, and invasion [1]. Genomic aberrations of Bcl6 can lead to the development of GC-derived lymphomas and it is considered a key oncogene in various cancers [2,5].
In mouse models, deletion of Bcl6 in lymphoid tissue inducer-like ILC3s resulted in dysregulation of the transcriptional program and enhanced expression of interleukin-17A (IL-17A) and IL-17F, associated with worsened inflammation in a colitis model, indicating its role in ILC3 subset biology [4]. In gastrointestinal stromal tumor (GIST) cells, induction of Bcl6 by imatinib mesylate (IM) treatment led to suppression of apoptosis and IM resistance. Targeting Bcl6 with an inhibitor, BI-3802, restored IM sensitivity and showed synergy with IM in vitro and in vivo, suggesting Bcl6's role in IM resistance [3].
In conclusion, Bcl6 is a critical regulator in immunity and tumorigenesis. Its functions in immune cell development, especially in GCs, are well-established. In disease areas such as lymphoma, GIST, and pre-eclampsia, studies using gene knockout or conditional knockout mouse models have revealed its roles in promoting tumor progression, drug resistance, and placental/endometrial pathophysiology, providing potential therapeutic targets for these diseases [1,2,3].
References:
1. Louwen, Frank, Kreis, Nina-Naomi, Ritter, Andreas, Solbach, Christine, Yuan, Juping. . BCL6, a key oncogene, in the placenta, pre-eclampsia and endometriosis. In Human reproduction update, 28, 890-909. doi:10.1093/humupd/dmac027. https://pubmed.ncbi.nlm.nih.gov/35640966/
2. Leeman-Neill, Rebecca J, Bhagat, Govind. 2018. BCL6 as a therapeutic target for lymphoma. In Expert opinion on therapeutic targets, 22, 143-152. doi:10.1080/14728222.2018.1420782. https://pubmed.ncbi.nlm.nih.gov/29262721/
3. Zeng, Xiangyu, Zhao, Fei, Jia, Jie, Lou, Zhenkun, Zhang, Peng. . Targeting BCL6 in Gastrointestinal Stromal Tumor Promotes p53-Mediated Apoptosis to Enhance the Antitumor Activity of Imatinib. In Cancer research, 83, 3624-3635. doi:10.1158/0008-5472.CAN-23-0082. https://pubmed.ncbi.nlm.nih.gov/37556508/
4. Tachó-Piñot, Roser, Stamper, Christopher T, King, James I, Withers, David R, Hepworth, Matthew R. 2023. Bcl6 is a subset-defining transcription factor of lymphoid tissue inducer-like ILC3. In Cell reports, 42, 113425. doi:10.1016/j.celrep.2023.113425. https://pubmed.ncbi.nlm.nih.gov/37950867/
5. McLachlan, Tabitha, Matthews, William C, Jackson, Evangeline R, Germon, Zacary P, Dun, Matthew D. . B-cell Lymphoma 6 (BCL6): From Master Regulator of Humoral Immunity to Oncogenic Driver in Pediatric Cancers. In Molecular cancer research : MCR, 20, 1711-1723. doi:10.1158/1541-7786.MCR-22-0567. https://pubmed.ncbi.nlm.nih.gov/36166198/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen