C57BL/6JCya-Hyou1em1flox/Cya
Common Name:
Hyou1-flox
Product ID:
S-CKO-01484
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Hyou1-flox
Strain ID
CKOCMP-12282-Hyou1-B6J-VA
Gene Name
Product ID
S-CKO-01484
Gene Alias
CBP-140; Cab140; Grp170; Orp150
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hyou1em1flox/Cya mice (Catalog S-CKO-01484) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000066601
NCBI RefSeq
NM_021395
Target Region
Exon 9~14
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
HYOU1, also known as Orp150, is an endoplasmic reticulum molecular chaperone protein. Various stimuli can trigger an unfolded protein response to endoplasmic reticulum stress, accompanied by the up-regulation of HYOU1 expression [2]. HYOU1 is involved in multiple biological processes and associated with many diseases.
In the context of intestinal injury, Fut2-dependent fucosylation of HYOU1 at the N-glycosylation site of asparagine (Asn) 862 was found to protect intestinal stem cells (ISCs) against inflammatory injury. Fut2ΔISC (ISC-specific Fut2 knockout) mouse models demonstrated that FUT2 depletion in ISCs aggravated epithelial damage and disrupted ISC growth and proliferation by escalating LPS-induced endoplasmic reticulum (ER) stress and initiating the IRE1/TRAF2/ASK1/JNK branch of the unfolded protein response (UPR) [1].
In conclusion, HYOU1 plays a crucial role in protecting cells from stress-related damage, as seen in the protection of ISCs against inflammatory injury. The use of gene-knockout mouse models, like the Fut2ΔISC mice, has been instrumental in uncovering these functions, providing insights into potential therapeutic strategies for intestinal injury disorders.
References：
1. Wang, Zhe, Tan, Chen, Duan, Caihan, Han, Chaoqun, Hou, Xiaohua. 2023. FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response. In Redox biology, 60, 102618. doi:10.1016/j.redox.2023.102618. https://pubmed.ncbi.nlm.nih.gov/36724577/
2. Rao, Shan, Oyang, Linda, Liang, Jiaxin, Zhou, Yujuan, Liao, Qianjin. 2021. Biological Function of HYOU1 in Tumors and Other Diseases. In OncoTargets and therapy, 14, 1727-1735. doi:10.2147/OTT.S297332. https://pubmed.ncbi.nlm.nih.gov/33707955/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen