C57BL/6JCya-Chi3l1em1flox/Cya
Common Name
Chi3l1-flox
Product ID
S-CKO-01719
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-12654-Chi3l1-B6J-VA
Status
When using this mouse strain in a publication, please cite “Chi3l1-flox Mouse (Catalog S-CKO-01719) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Chi3l1-flox
Strain ID
CKOCMP-12654-Chi3l1-B6J-VA
Gene Name
Product ID
S-CKO-01719
Gene Alias
Gp39, Brp39, Chil1
Background
C57BL/6JCya
Gene Full Name
chitinase-like 1
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 1 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000082060
NCBI Transcript ID
NM_007695
Target Region
Exon 3~5
Size of Effective Region
~2.3 kb
Overview of Gene Research
Chi3l1, also known as chitinase-3-like protein 1 or YKL-40, belongs to glycoside hydrolase family 18. It binds to chitin, heparin, and hyaluronic acid. Chi3l1 is synthesized and secreted by various cells and plays major roles in tissue injury, inflammation, repair, and remodeling. It is involved in multiple biological processes and is associated with numerous diseases [4].
In triple-negative breast cancer (TNBC), Chi3l1 ablation in the PyMT breast cancer model generated an anti-tumor immune response, delayed tumor onset, increased T-cell infiltration, and improved response to immune-checkpoint blockade. Mechanistically, Chi3l1 promoted neutrophil recruitment and neutrophil extracellular trap formation, which blocked T-cell infiltration [1].
In the context of Alzheimer's disease, Chi3l1 deletion in mice decreased amyloid plaque burden in the APP/PS1 model, suggesting that Chi3l1 may suppress glial phagocytic activation and promote amyloid accumulation. Also, Chi3l1 expression is regulated by the circadian clock [2].
In multiple sclerosis, astrocytic deletion of Chi3l1 mitigated neurogenic deficits and cognitive dysfunction. CHI3L1 interacts with CRTH2/RAGE, attenuating β-catenin signaling [3].
In conclusion, Chi3l1 is involved in diverse biological processes. Gene-knockout mouse models have revealed its roles in cancer, neurodegenerative diseases like Alzheimer's, and multiple sclerosis. These studies help us understand the disease mechanisms and may provide potential therapeutic targets for these diseases.
References：
1. Taifour, Tarek, Attalla, Sherif Samer, Zuo, Dongmei, Park, Morag, Muller, William J. 2023. The tumor-derived cytokine Chi3l1 induces neutrophil extracellular traps that promote T cell exclusion in triple-negative breast cancer. In Immunity, 56, 2755-2772.e8. doi:10.1016/j.immuni.2023.11.002. https://pubmed.ncbi.nlm.nih.gov/38039967/
2. Lananna, Brian V, McKee, Celia A, King, Melvin W, Cruchaga, Carlos, Musiek, Erik S. . Chi3l1/YKL-40 is controlled by the astrocyte circadian clock and regulates neuroinflammation and Alzheimer's disease pathogenesis. In Science translational medicine, 12, . doi:10.1126/scitranslmed.aax3519. https://pubmed.ncbi.nlm.nih.gov/33328329/
3. Song, Yanna, Jiang, Wei, Afridi, Shabbir Khan, Qiu, Wei, Tang, Changyong. 2024. Astrocyte-derived CHI3L1 signaling impairs neurogenesis and cognition in the demyelinated hippocampus. In Cell reports, 43, 114226. doi:10.1016/j.celrep.2024.114226. https://pubmed.ncbi.nlm.nih.gov/38733586/
4. Zhao, Ting, Su, Zhongping, Li, Yingchang, Zhang, Xiaoren, You, Qiang. 2020. Chitinase-3 like-protein-1 function and its role in diseases. In Signal transduction and targeted therapy, 5, 201. doi:10.1038/s41392-020-00303-7. https://pubmed.ncbi.nlm.nih.gov/32929074/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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