C57BL/6JCya-Cyp11a1em1flox/Cya
Common Name:
Cyp11a1-flox
Product ID:
S-CKO-01956
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cyp11a1-flox
Strain ID
CKOCMP-13070-Cyp11a1-B6J-VA
Gene Name
Product ID
S-CKO-01956
Gene Alias
Cyp11a; Cypxia1; D9Ertd411e; P450scc; Scc; cscc
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cyp11a1em1flox/Cya mice (Catalog S-CKO-01956) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034874
NCBI RefSeq
NM_019779
Target Region
Exon 3
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Cyp11a1, encoding the P450scc enzyme, catalyzes the first and rate-limiting step of steroid biosynthesis [3]. It is expressed in the adrenals, gonads, placenta, and skin [3,4,6]. The promoter of the Cyp11a1 gene binds to transcription factor SF-1, playing a crucial role in its tissue-specific and hormonally regulated expression [3,5,6]. The gene is essential for steroidogenesis, which is vital for physiological regulation, including glucose and salt balance, sexual characteristics, and neurosteroid production [2,5]. Animal models like mice and zebrafish are valuable for studying Cyp11a1 gene regulatory pathways related to steroid imbalance [3].
In a study on polycystic ovarian syndrome (PCOS), artemisinins were found to promote Cyp11a1 protein degradation by enhancing its interaction with LONP1, thus curbing hyperandrogenemia in rodent models and human patients [1]. In transgenic mouse experiments, the importance of the SF-1-binding site in the Cyp11a1 promoter for balanced gene expression was demonstrated, and targeted mutagenesis of Cyp11a1 revealed its significance in steroid secretion, gene regulation, and genitalia development [3].
In conclusion, Cyp11a1 is fundamental for steroid biosynthesis and has a significant impact on various physiological processes. Model-based research, especially mouse models, has provided insights into its role in diseases like PCOS and in key processes such as steroid secretion and gene regulation, which helps in understanding the underlying mechanisms and developing potential therapies.
References:
1. Liu, Yang, Jiang, Jing-Jing, Du, Shao-Yue, Xu, Cong-Jian, Tang, Qi-Qun. 2024. Artemisinins ameliorate polycystic ovarian syndrome by mediating LONP1-CYP11A1 interaction. In Science (New York, N.Y.), 384, eadk5382. doi:10.1126/science.adk5382. https://pubmed.ncbi.nlm.nih.gov/38870290/
2. Lin, Yiqi Christina, Papadopoulos, Vassilios. 2021. Neurosteroidogenic enzymes: CYP11A1 in the central nervous system. In Frontiers in neuroendocrinology, 62, 100925. doi:10.1016/j.yfrne.2021.100925. https://pubmed.ncbi.nlm.nih.gov/34015388/
3. Hu, Meng-Chun, Hsu, Hwei-Jan, Guo, Ing-Cherng, Chung, Bon-Chu. . Function of Cyp11a1 in animal models. In Molecular and cellular endocrinology, 215, 95-100. doi:. https://pubmed.ncbi.nlm.nih.gov/15026180/
4. Tongkao-On, Wannit, Carter, Sally, Reeve, Vivienne E, Tuckey, Robert C, Mason, Rebecca S. 2014. CYP11A1 in skin: an alternative route to photoprotection by vitamin D compounds. In The Journal of steroid biochemistry and molecular biology, 148, 72-8. doi:10.1016/j.jsbmb.2014.11.015. https://pubmed.ncbi.nlm.nih.gov/25448743/
5. Guo, Ing-Cherng, Hu, Meng-Chun, Chung, Bon-Chu. . Transcriptional regulation of CYP11A1. In Journal of biomedical science, 10, 593-8. doi:. https://pubmed.ncbi.nlm.nih.gov/14576461/
6. Shih, Meng-Chun Monica, Chiu, Yi-Ning, Hu, Meng-Chun, Guo, Ing-Chern, Chung, Bon-chu. 2010. Regulation of steroid production: analysis of Cyp11a1 promoter. In Molecular and cellular endocrinology, 336, 80-4. doi:10.1016/j.mce.2010.12.017. https://pubmed.ncbi.nlm.nih.gov/21195129/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen