C57BL/6JCya-Cyp19a1em1flox/Cya
Common Name:
Cyp19a1-flox
Product ID:
S-CKO-01959
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cyp19a1-flox
Strain ID
CKOCMP-13075-Cyp19a1-B6J-VA
Gene Name
Product ID
S-CKO-01959
Gene Alias
Ar; ArKO; Cyp19; Int-5; Int5; p450arom
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cyp19a1em1flox/Cya mice (Catalog S-CKO-01959) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034811
NCBI RefSeq
NM_007810
Target Region
Exon 3
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Cyp19a1, also known as aromatase, is a member of cytochrome P450 family of heme-thiolate monooxygenases. It converts androgens into estrogens, which is crucial for female sexual development as well as growth and development in both sexes. This process is part of the steroid biosynthesis pathway, and Cyp19a1 depends on reducing equivalents from the reduced nicotinamide adenine dinucleotide phosphate via the cytochrome P450 oxidoreductase coded by POR [3].
In a study of the Chinese Han population, the contribution of Cyp19a1 polymorphisms to coronary heart disease (CHD) susceptibility was found to be associated with age, gender, and clinical phenotypes (course of the disease and Gensini score) [1]. In a case-control study and meta-analysis in China, the Cyp19a1 rs700519 polymorphism was related to breast tumorigenesis, especially showing a significant negative relationship with risk and disease-free survival rates in postmenopausal hormone receptor-positive patients [2]. Also, in pregnant women using selective serotonin reuptake inhibitors (SSRIs), the enzyme activity of Cyp19a1 in placental microsomal fractions was lower compared to controls, which may lead to disturbances in maternal steroid hormone balance [4]. A study on endometriosis found that while there was no difference in Cyp19a1 mRNA expression in luteinized granulosa cells between women with stage III-IV endometriosis and controls, the correlation between Cyp19a1 expression and follicular estradiol levels, as well as the number of oocytes retrieved, was different between the two groups, highlighting a potential mechanism affecting ovulation in endometriosis patients [5].
In conclusion, Cyp19a1 is essential for estrogen synthesis and thus for sexual and general development. Studies on its polymorphisms and expression changes have revealed its associations with diseases such as coronary heart disease, breast cancer, and endometriosis, as well as its role in placental function during pregnancy. These findings contribute to our understanding of the biological functions of Cyp19a1 and its implications in various disease conditions.
References:
1. Ye, Wei, Tang, Qizhu, Wang, Lei, He, Qi, Peng, Kaiwei. 2022. Contribution of CYP19A1, CYP1A1, and CYP1A2 polymorphisms in coronary heart disease risk among the Chinese Han population. In Functional & integrative genomics, 22, 515-524. doi:10.1007/s10142-022-00850-y. https://pubmed.ncbi.nlm.nih.gov/35380334/
2. Lv, Fei, Huang, Wanying, Wang, Ying. . The CYP19A1 rs700519 Polymorphism and Breast Cancer Susceptibility in China: A Case-Control Study and Updated Meta-Analysis. In Genetic testing and molecular biomarkers, 25, 486-495. doi:10.1089/gtmb.2021.0032. https://pubmed.ncbi.nlm.nih.gov/34280004/
3. Parween, Shaheena, DiNardo, Giovanna, Baj, Francesca, Gilardi, Gianfranco, Pandey, Amit V. 2019. Differential effects of variations in human P450 oxidoreductase on the aromatase activity of CYP19A1 polymorphisms R264C and R264H. In The Journal of steroid biochemistry and molecular biology, 196, 105507. doi:10.1016/j.jsbmb.2019.105507. https://pubmed.ncbi.nlm.nih.gov/31669572/
4. Sahlman, H, Itkonen, A, Lehtonen, M, Keski-Nisula, L, Rysä, J. 2022. Altered activities of CYP1A1 and CYP19A1 enzymes in women using SSRI medication during pregnancy. In Placenta, 129, 30-35. doi:10.1016/j.placenta.2022.09.013. https://pubmed.ncbi.nlm.nih.gov/36198245/
5. Giacomini, Elisa, Pagliardini, Luca, Minetto, Sabrina, Somigliana, Edgardo, Viganò, Paola. 2023. The relationship between CYP19A1 gene expression in luteinized granulosa cells and follicular estradiol output in women with endometriosis. In The Journal of steroid biochemistry and molecular biology, 237, 106439. doi:10.1016/j.jsbmb.2023.106439. https://pubmed.ncbi.nlm.nih.gov/38048918/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen