C57BL/6JCya-Cyp2j5em1flox/Cya
Common Name:
Cyp2j5-flox
Product ID:
S-CKO-01973
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Cyp2j5-flox
Strain ID
CKOCMP-13109-Cyp2j5-B6J-VA
Gene Name
Product ID
S-CKO-01973
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cyp2j5em1flox/Cya mice (Catalog S-CKO-01973) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000030299
NCBI RefSeq
NM_010007
Target Region
Exon 4
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Cyp2j5 is a cytochrome P450 enzyme that is abundant in the mouse kidney. It is active in the metabolism of arachidonic acid to epoxyeicosatrienoic acids (EETs), which are involved in modulating sodium transport, vascular tone, and fluid/electrolyte transport, as well as mediating hormonal action in the kidney [4]. It may also be associated with the metabolism of small molecules like flavanone, DHEA, and 7-ethoxycoumarin [6].
In Cyp2j5-/-mice, acetylcholine-induced relaxation was different from that in C57Bl/6 mice, and it depended on nitric oxide rather than CYP-epoxygenases. Angiotensin-II reduced acetylcholine-and NECA-induced relaxation in both Cyp2j5-/-and C57Bl/6 mice, and there was a gender-specific difference in NECA-induced response in Cyp2j5-/-mice when treated with Ang-II [1]. Female Cyp2j5-/-mice had increased blood pressure, enhanced proximal tubular transport rates, and exaggerated afferent arteriolar responses to angiotensin II and endothelin I, and these effects were related to reduced plasma 17β-estradiol levels [5]. Also, deletion of Cyp2j5 led to decreased EET production in the kidney [5]. In lipopolysaccharide-induced acute lung injury in mice, the mRNA expression of Cyp2j5 was decreased [2]. The renal expression of Cyp2j5 is up-regulated by androgen and down-regulated by estrogen [3].
In conclusion, Cyp2j5 plays crucial roles in the regulation of vascular relaxation, blood pressure, and proximal tubular function, with gender-specific differences. Its dysregulation is associated with conditions like acute lung injury. Gene knockout mouse models have been instrumental in revealing these functions of Cyp2j5 in the context of these biological processes and disease-related phenotypes [1,2,3,5].
References:
1. Agba, Stephanie, Hanif, Ahmad, Edin, Matthew L, Zeldin, Darryl C, Nayeem, Mohammed A. 2020. Cyp2j5-Gene Deletion Affects on Acetylcholine and Adenosine-Induced Relaxation in Mice: Role of Angiotensin-II and CYP-Epoxygenase Inhibitor. In Frontiers in pharmacology, 11, 27. doi:10.3389/fphar.2020.00027. https://pubmed.ncbi.nlm.nih.gov/32116704/
2. Yang, Hui-Hui, Duan, Jia-Xi, Liu, Shao-Kun, Zhou, Yong, Guan, Cha-Xiang. 2020. A COX-2/sEH dual inhibitor PTUPB alleviates lipopolysaccharide-induced acute lung injury in mice by inhibiting NLRP3 inflammasome activation. In Theranostics, 10, 4749-4761. doi:10.7150/thno.43108. https://pubmed.ncbi.nlm.nih.gov/32308747/
3. Ma, Jixiang, Graves, Joan, Bradbury, J Alyce, Korach, Kenneth S, Zeldin, Darryl C. . Regulation of mouse renal CYP2J5 expression by sex hormones. In Molecular pharmacology, 65, 730-43. doi:. https://pubmed.ncbi.nlm.nih.gov/14978252/
4. Ma, J, Qu, W, Scarborough, P E, Breyer, M D, Zeldin, D C. . Molecular cloning, enzymatic characterization, developmental expression, and cellular localization of a mouse cytochrome P450 highly expressed in kidney. In The Journal of biological chemistry, 274, 17777-88. doi:. https://pubmed.ncbi.nlm.nih.gov/10364221/
5. Athirakul, Krairerk, Bradbury, J Alyce, Graves, Joan P, Coffman, Thomas M, Zeldin, Darryl C. 2008. Increased blood pressure in mice lacking cytochrome P450 2J5. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 22, 4096-108. doi:10.1096/fj.08-114413. https://pubmed.ncbi.nlm.nih.gov/18716027/
6. Uno, Tomohide, Okamoto, Sota, Masuda, Satoko, Yamagata, Hiroshi, Imaishi, Hiromasa. . Bioconversion of small molecules by cytochrome P450 species expressed in Escherichia coli. In Biotechnology and applied biochemistry, 50, 165-71. doi:. https://pubmed.ncbi.nlm.nih.gov/18052937/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen