C57BL/6JCya-Edn2em1flox/Cya
Common Name:
Edn2-flox
Product ID:
S-CKO-02141
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Edn2-flox
Strain ID
CKOCMP-13615-Edn2-B6J-VB
Gene Name
Product ID
S-CKO-02141
Gene Alias
ET-2; PPET2; VIC
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Edn2em1flox/Cya mice (Catalog S-CKO-02141) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000030384
NCBI RefSeq
NM_007902
Target Region
Exon 2
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Edn2, also known as endothelin-2, is a 21-amino-acid-long peptide-encoding gene. It is involved in multiple physiological processes.
In the ovary, it plays an essential role in ovulation by promoting contraction in the myofibroblast cells of the theca layer of the follicle [2,5]. In the retina, it is related to photoreceptor protection and may be involved in the regulation of diabetic retinopathy [1,6].
In mouse models, in Edn2(-/-) mice carrying homozygous Pde6b(rd1) alleles or the Tg(RHO P347S) transgene, photoreceptor (PR) survival increased, suggesting that systemic EDN2 loss of function affected mutant PR survival. Restoring Edn2 expression in Pde6b(rd1/rd1); Edn2(-/-) PRs increased PR survival, indicating that increased Edn2 expression is protective to mutant PRs [3]. In conditional Edn2 knockout (Edn2-iKO) mice used as an in vivo acute lung injury (ALI) model, Edn2 deletion suppressed acute lung inflammation by reducing neutrophil-mediated IFNγ/STAT1/IL-1β/NFĸB signaling cascade [4].
In conclusion, Edn2 is crucial for ovulation, photoreceptor protection, and may be involved in the regulation of ALI and diabetic retinopathy. The gene knockout and conditional knockout mouse models have revealed its role in these biological processes and disease conditions, providing insights into related disease mechanisms and potential treatment targets.
References：
1. Iwagawa, Toshiro, Aihara, Yuko, Umutoni, Daisy, Miyado, Kenji, Watanabe, Sumiko. . Cd9 Protects Photoreceptors from Injury and Potentiates Edn2 Expression. In Investigative ophthalmology & visual science, 61, 7. doi:10.1167/iovs.61.3.7. https://pubmed.ncbi.nlm.nih.gov/32150249/
2. Ko, CheMyong J, Cho, Yoon Min, Ham, Eugene, Cacioppo, Joseph A, Park, Chan Jin. 2022. Endothelin 2: a key player in ovulation and fertility. In Reproduction (Cambridge, England), 163, R71-R80. doi:10.1530/REP-21-0313. https://pubmed.ncbi.nlm.nih.gov/35167488/
3. Bramall, Alexa N, Szego, Michael J, Pacione, Laura R, Yanagisawa, Masashi, McInnes, Roderick R. 2013. Endothelin-2-mediated protection of mutant photoreceptors in inherited photoreceptor degeneration. In PloS one, 8, e58023. doi:10.1371/journal.pone.0058023. https://pubmed.ncbi.nlm.nih.gov/23469133/
4. Musthafa, Ahmad, Ryanto, Gusty Rizky Teguh, Suraya, Ratoe, Hirata, Ken-Ichi, Emoto, Noriaki. 2023. Acute Amelioration of Inflammatory Activity Caused by Endothelin-2 Deficiency during Acute Lung Injury. In The Kobe journal of medical sciences, 69, E96-E105. doi:10.24546/0100483406. https://pubmed.ncbi.nlm.nih.gov/37941117/
5. Ko, Chemyong, Gieske, Mary C, Al-Alem, Linah, Iglarz, Marc, Koo, Yongbum. 2006. Endothelin-2 in ovarian follicle rupture. In Endocrinology, 147, 1770-9. doi:. https://pubmed.ncbi.nlm.nih.gov/16410304/
6. Binz, Nicolette, Rakoczy, Elizabeth P, Ali Rahman, Ireni S, Vagaja, Nermina N, Lai, Chooi-May. 2016. Biomarkers for Diabetic Retinopathy - Could Endothelin 2 Be Part of the Answer? In PloS one, 11, e0160442. doi:10.1371/journal.pone.0160442. https://pubmed.ncbi.nlm.nih.gov/27482904/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen