C57BL/6JCya-F8aem1flox/Cya
Common Name:
F8a-flox
Product ID:
S-CKO-02326
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
F8a-flox
Strain ID
CKOCMP-14070-F8a-B6J-VA
Gene Name
Product ID
S-CKO-02326
Gene Alias
DXUcsf1; F8a1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-F8aem1flox/Cya mice (Catalog S-CKO-02326) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000105111
NCBI RefSeq
NM_007978
Target Region
Exon 1
Size of Effective Region
~3.0 kb
Detailed Document
Overview of Gene Research
F8a, a DNA sequence, has significance in multiple biological contexts.
In the context of hemophilia A, a copy of the F8A gene is located within intron 22 of the factor VIII (FVIII) coagulation gene, with two others 500 kb upstream. Homologous recombination between the intragenic F8A gene copy and either the distal or proximal copy leads to severe hemophilia A as no intact FVIII is produced [1].
In immunohistochemical studies comparing clear cell sarcoma of the kidney (CCSK) and Wilms' tumor (WT), CCSK cells were negative for Factor-8 related antigen (F8A), while neither blastemal, stromal nor epithelial elements in WT were positive for F8A. This can potentially aid in differentiating between these two tumors with different prognoses and treatment requirements [2].
In conclusion, F8a is associated with the molecular pathogenesis of hemophilia A through its role in the FVIII gene inversion mechanism. In tumor-related studies, the absence of F8A expression in specific cell types of CCSK and WT can be a useful diagnostic feature. Understanding F8a's functions in these disease conditions helps in developing more targeted diagnostic and treatment strategies.
References：
1. De Brasi, C D, Slavutsky, I R, Larripa, I B. . [Molecular genetics of hemophilia A]. In Medicina, 56, 509-17. doi:. https://pubmed.ncbi.nlm.nih.gov/9239887/
2. Looi, L M, Cheah, P L. . An immunohistochemical study comparing clear cell sarcoma of the kidney and Wilms' tumor. In Pathology, 25, 106-9. doi:. https://pubmed.ncbi.nlm.nih.gov/8396229/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen