C57BL/6NCya-Fcgrtem1flox/Cya
Common Name:
Fcgrt-flox
Product ID:
S-CKO-02381
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Fcgrt-flox
Strain ID
CKOCMP-14132-Fcgrt-B6N-VA
Gene Name
Product ID
S-CKO-02381
Gene Alias
FcRn
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Fcgrtem1flox/Cya mice (Catalog S-CKO-02381) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000003512
NCBI RefSeq
NM_010189
Target Region
Exon 5~7
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
Fcgrt, also known as the gene encoding the neonatal Fc receptor (FcRn) α-chain, is crucial for the trafficking of endogenous and exogenous IgG molecules and albumin in various tissues [6]. FcRn binds to IgG, protecting it from lysosomal degradation by transporting it back to the cell surface for re-entry into the circulation, thus extending the serum IgG lifespan. This process is involved in the function of IVIg and is relevant to the pharmacokinetics of therapeutic monoclonal antibodies [1,8]. Animal models like rhesus macaques, which have VNTR polymorphisms in Fcgrt affecting IgG levels, can be potential models for studying the immunological response related to Fcgrt [2,7].
In humans, Fcgrt polymorphisms, such as the VNTR3/2 in myasthenia gravis patients, may affect the duration of infused IgG in the circulation and IVIg effectiveness [1]. Also, in enterovirus B infection, FcRn binds to the virus particles through its FCGRT subunit, acting as the uncoating receptor, which provides a structural basis for understanding enterovirus entry mechanisms [3]. DNA methylation in regulatory regions of Fcgrt contributes to FcRn expression, and microRNA-3181 is an epigenetic regulator of Fcgrt expression [4,6]. In Indian water buffaloes, Fcgrt polymorphisms are associated with colostral IgG concentration, which is important for neonatal passive immunity [5].
In conclusion, Fcgrt plays a vital role in IgG and albumin trafficking, influencing the effectiveness of immunotherapies, the course of certain infections, and neonatal immunity. Studies on Fcgrt using genetic models help in understanding its function in various biological processes and disease conditions, providing insights for the development of more effective treatments related to IgG-based therapies and infectious diseases [1-9].
References:
1. Dalakas, Marinos C, Spaeth, Peter J. 2021. The importance of FcRn in neuro-immunotherapies: From IgG catabolism, FCGRT gene polymorphisms, IVIg dosing and efficiency to specific FcRn inhibitors. In Therapeutic advances in neurological disorders, 14, 1756286421997381. doi:10.1177/1756286421997381. https://pubmed.ncbi.nlm.nih.gov/33717213/
2. Pavlova, L E, Timina, M F, Agumava, A A, Panchenko, A V. 2024. Rhesus Macaques: VNTR Polymorphism of the FCGRT Gene. In Bulletin of experimental biology and medicine, 177, 379-382. doi:10.1007/s10517-024-06193-x. https://pubmed.ncbi.nlm.nih.gov/39134810/
3. Zhao, Xin, Zhang, Guigen, Liu, Sheng, Wei, Wensheng, Gao, George F. 2019. Human Neonatal Fc Receptor Is the Cellular Uncoating Receptor for Enterovirus B. In Cell, 177, 1553-1565.e16. doi:10.1016/j.cell.2019.04.035. https://pubmed.ncbi.nlm.nih.gov/31104841/
4. Cejas, R B, Ferguson, D C, Quiñones-Lombraña, A, Bard, J E, Blanco, J G. 2019. Contribution of DNA methylation to the expression of FCGRT in human liver and myocardium. In Scientific reports, 9, 8674. doi:10.1038/s41598-019-45203-1. https://pubmed.ncbi.nlm.nih.gov/31209240/
5. Agrawal, Swati, Kumar, Subodh, Chaudhary, Rajni, Kumar, Amit, Sivamani, B. 2022. Detection of nucleotide variants in FCGRT (Fc fragment of IgG, receptor, transporter, alpha) gene and their influence on colostral IgG concentration in Indian water buffalo (Bubalus bubalis). In Molecular biology reports, 49, 3773-3781. doi:10.1007/s11033-022-07217-3. https://pubmed.ncbi.nlm.nih.gov/35152368/
6. Ferguson, Daniel C, Blanco, Javier G. 2018. Regulation of the Human Fc-Neonatal Receptor alpha-Chain Gene FCGRT by MicroRNA-3181. In Pharmaceutical research, 35, 15. doi:10.1007/s11095-017-2294-0. https://pubmed.ncbi.nlm.nih.gov/29302759/
7. Shubin, Zhanna, Tagaya, Yutaka, Poonia, Bhawna. 2017. Functional polymorphisms in rhesus macaque FCGRT and β2-m. In Immunogenetics, 70, 179-183. doi:10.1007/s00251-017-1022-6. https://pubmed.ncbi.nlm.nih.gov/28785825/
8. Passot, Christophe, Azzopardi, Nicolas, Renault, Sylvaine, Paintaud, Gilles, Gouilleux-Gruart, Valérie. 2013. Influence of FCGRT gene polymorphisms on pharmacokinetics of therapeutic antibodies. In mAbs, 5, 614-9. doi:10.4161/mabs.24815. https://pubmed.ncbi.nlm.nih.gov/23751752/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen