C57BL/6JCya-Fgf7em1flox/Cya
Common Name:
Fgf7-flox
Product ID:
S-CKO-02410
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Fgf7-flox
Strain ID
CKOCMP-14178-Fgf7-B6J-VA
Gene Name
Product ID
S-CKO-02410
Gene Alias
Fgf5b; Kgf
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fgf7em1flox/Cya mice (Catalog S-CKO-02410) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000064794
NCBI RefSeq
NM_008008
Target Region
Exon 3
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Fgf7, also known as keratinocyte growth factor (KGF), is a member of the fibroblast growth factor family. It plays crucial roles in various biological processes. Specifically, it is involved in unidirectional mesenchyme-to-epithelium signaling, which is essential for the development of most organs, glands, and limbs. Fgf7 specifically activates the "b" isoforms of FGFR1 (FGFR1b) and FGFR2 (FGFR2b) by engaging in specific contacts with two alternatively spliced loop regions in the immunoglobulin-like domain 3 (D3) of these receptors [2].
Single-cell RNA-seq analysis revealed that Fgf7 mediates a novel interaction between muscle fibro-adipogenic progenitors (FAPs) and satellite cells. Exogenous Fgf7 can augment the proliferation of satellite cells, which benefits muscle regeneration and counteracts age-related myopathy [1]. In the context of COVID-19, Fgf7 enhances the expression of ACE2 in human islet organoids, facilitating SARS-CoV-2 infection and resulting in impaired insulin secretion [3]. In rhabdomyosarcomas, Fgf7-FGFR2 autocrine signaling increases growth and chemoresistance of fusion-positive cases [4]. In ovarian cancer, cancer-associated fibroblast-secreted Fgf7 promotes cancer progression by inhibiting the ubiquitination and degradation of HIF-1α via FGFR2 interaction [5].
In conclusion, Fgf7 is involved in multiple biological processes, including organ development, muscle regeneration, and cell-cell communication. Its role in diseases such as age-related myopathy, COVID-19-associated diabetes, rhabdomyosarcomas, and ovarian cancer has been revealed through various studies. Understanding the function of Fgf7 provides potential therapeutic targets for these diseases.
References:
1. Ma, Lu, Meng, Yingying, An, Yalong, Yang, Gongshe, Li, Xiao. 2024. Single-cell RNA-seq reveals novel interaction between muscle satellite cells and fibro-adipogenic progenitors mediated with FGF7 signalling. In Journal of cachexia, sarcopenia and muscle, 15, 1388-1403. doi:10.1002/jcsm.13484. https://pubmed.ncbi.nlm.nih.gov/38751367/
2. Zinkle, Allen, Mohammadi, Moosa. 2019. Structural Biology of the FGF7 Subfamily. In Frontiers in genetics, 10, 102. doi:10.3389/fgene.2019.00102. https://pubmed.ncbi.nlm.nih.gov/30809251/
3. Meng, Hao, Liao, Zhiying, Ji, Yanting, Xu, Tao, Liu, Huisheng. 2024. FGF7 enhances the expression of ACE2 in human islet organoids aggravating SARS-CoV-2 infection. In Signal transduction and targeted therapy, 9, 104. doi:10.1038/s41392-024-01790-8. https://pubmed.ncbi.nlm.nih.gov/38654010/
4. Milton, Christopher I, Selfe, Joanna, Aladowicz, Ewa, Kirkin, Vladimir, Shipley, Janet M. 2021. FGF7-FGFR2 autocrine signaling increases growth and chemoresistance of fusion-positive rhabdomyosarcomas. In Molecular oncology, 16, 1272-1289. doi:10.1002/1878-0261.13145. https://pubmed.ncbi.nlm.nih.gov/34850536/
5. Feng, Songwei, Ding, Bo, Dai, Zhu, Xiao, Zhongdang, Shen, Yang. 2024. Cancer-associated fibroblast-secreted FGF7 as an ovarian cancer progression promoter. In Journal of translational medicine, 22, 280. doi:10.1186/s12967-024-05085-y. https://pubmed.ncbi.nlm.nih.gov/38491511/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen