C57BL/6JCya-Ccn2em1flox/Cya
Common Name:
Ccn2-flox
Product ID:
S-CKO-02432
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ccn2-flox
Strain ID
CKOCMP-14219-Ccn2-B6J-VA
Gene Name
Product ID
S-CKO-02432
Gene Alias
Ctgf; Fisp12; Hcs24; fisp-12
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ccn2em1flox/Cya mice (Catalog S-CKO-02432) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020171
NCBI RefSeq
NM_010217
Target Region
Exon 1~5
Size of Effective Region
~3.3 kb
Detailed Document
Overview of Gene Research
Ccn2, also known as connective tissue growth factor (CTGF), is a matricellular protein. It plays diverse roles in biological processes, regulating cell growth, invasion, and migration. Ccn2 is involved in multiple pathways such as TGF-β, TGFBR/SMAD, ILK and Syk signaling cascades, and is crucial for the development of tissues like cartilage and bone in the skeletal system [1,2,4,5].
A monoallelic variant in CCN2 causes autosomal dominant spondyloepimetaphyseal dysplasia with low bone mass. Osteoblast lineage-specific Ccn2-deficient mice (Ccn2fl/fl;Prx1Cre) recapitulated phenotypes including low bone mass, with pathological mechanisms involving decreased bone formation, increased bone resorption, and abnormal growth plate formation, highlighting the critical role of CCN2 in osteogenesis [3].
In conclusion, Ccn2 is essential for tissue development, especially in the skeletal system. The study of Ccn2-deficient mouse models has revealed its role in skeletal dysplasia, providing insights into the mechanisms of bone-related diseases and potential therapeutic targets.
References：
1. Ghosh, Priya, Dey, Ankita, Nandi, Suvendu, Das, Subhayan, Mandal, Mahitosh. 2025. CTGF (CCN2): a multifaceted mediator in breast cancer progression and therapeutic targeting. In Cancer metastasis reviews, 44, 32. doi:10.1007/s10555-025-10248-4. https://pubmed.ncbi.nlm.nih.gov/39945880/
2. Li, Ziyun, Jiang, Jia, Cai, Kangwen, Liu, Ning-Ning, Zhao, Jinzhong. 2025. CCN2 mediates fibroblast-macrophage interaction in knee arthrofibrosis based on single-cell RNA-seq analysis. In Bone research, 13, 26. doi:10.1038/s41413-025-00400-9. https://pubmed.ncbi.nlm.nih.gov/39994205/
3. Li, Shanshan, Shao, Rui, Li, Shufa, Zou, Weiguo, Zhang, Zhenlin. 2024. A monoallelic variant in CCN2 causes an autosomal dominant spondyloepimetaphyseal dysplasia with low bone mass. In Bone research, 12, 60. doi:10.1038/s41413-024-00364-2. https://pubmed.ncbi.nlm.nih.gov/39414788/
4. Liu, Shan-Chi, Hsieh, Hung-Lun, Tsai, Chun-Hao, Hsu, Chin-Jung, Tang, Chih-Hsin. 2022. CCN2 Facilitates IL-17 Production and Osteoclastogenesis in Human Osteoarthritis Synovial Fibroblasts by Inhibiting miR-655 Expression. In Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 37, 1944-1955. doi:10.1002/jbmr.4661. https://pubmed.ncbi.nlm.nih.gov/35876037/
5. Kubota, Satoshi, Takigawa, Masaharu. . Cellular and molecular actions of CCN2/CTGF and its role under physiological and pathological conditions. In Clinical science (London, England : 1979), 128, 181-96. doi:10.1042/CS20140264. https://pubmed.ncbi.nlm.nih.gov/25294165/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen