C57BL/6JCya-Flot2em1flox/Cya
Common Name:
Flot2-flox
Product ID:
S-CKO-02454
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Flot2-flox
Strain ID
CKOCMP-14252-Flot2-B6J-VA
Gene Name
Product ID
S-CKO-02454
Gene Alias
Esa; reggie-2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Flot2em1flox/Cya mice (Catalog S-CKO-02454) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000072289
NCBI RefSeq
NM_001040403
Target Region
Exon 4
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Flot2, also known as flotillin-2, is a lipid raft scaffold protein. It is thought to be involved in various biological processes such as endocytosis, neural differentiation, and may be related to multiple signaling pathways. It has shown importance in maintaining normal cellular functions and its dysregulation is associated with several diseases [3].
In sepsis, Flot2 deficiency in B cells, as shown by Flot2 knockout (Flot2-/-) mice, leads to up-regulation of effector B cell cytokines like Il6, Il1b and Cxcl10, aggravating sepsis with increased lung injury and shortened survival time. This indicates Flot2 is a controller of B cells and its deficiency amplifies inflammation in sepsis [1].
In proteinuric kidney disease, podocyte-specific Flot2 deletion worsens albuminuria, podocyte injury and glomerular pathology in mouse models, while overexpression prevents these, suggesting Flot2 stabilizes the podocin-nephrin complex in lipid rafts of podocytes [2].
In conclusion, Flot2 plays essential roles in maintaining normal B-cell-mediated immune responses and podocyte function. The Flot2 KO/CKO mouse models have been crucial in revealing its roles in sepsis and proteinuric kidney disease, providing insights into the pathogenesis of these diseases and potential therapeutic targets.
References:
1. Yang, Qin, Zhang, Zhenhua, Chen, Ziye, Wang, Junjian, Li, Xiaojuan. 2023. Flot2 deficiency facilitates B cell-mediated inflammatory responses and endotoxic shock. In Immunology, 170, 567-578. doi:10.1111/imm.13692. https://pubmed.ncbi.nlm.nih.gov/37688314/
2. Yu, Chunping, Zhang, Hong, Liu, Shuangxin, Zhang, Li, Shi, Wei. 2023. Flot2 acts as a novel mediator of podocyte injury in proteinuric kidney disease. In International journal of biological sciences, 19, 502-520. doi:10.7150/ijbs.78945. https://pubmed.ncbi.nlm.nih.gov/36632460/
3. Hanafusa, Kei, Hayashi, Nobuhiro. 2019. The Flot2 component of the lipid raft changes localization during neural differentiation of P19C6 cells. In BMC molecular and cell biology, 20, 38. doi:10.1186/s12860-019-0225-0. https://pubmed.ncbi.nlm.nih.gov/31455216/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen