C57BL/6JCya-Gabra4em1flox/Cya
Common Name
Gabra4-flox
Product ID
S-CKO-02533
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-14397-Gabra4-B6J-VA
When using this mouse strain in a publication, please cite “Gabra4-flox Mouse (Catalog S-CKO-02533) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Gabra4-flox
Strain ID
CKOCMP-14397-Gabra4-B6J-VA
Gene Name
Product ID
S-CKO-02533
Gene Alias
Gabra-4
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 5
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000031121
NCBI RefSeq
NM_010251
Target Region
Exon 3
Size of Effective Region
~0.6 kb
Overview of Gene Research
Gabra4, encoding the α4 -subunit of GABAA receptors (GABAARs), is involved in synaptic transmission within the GABAergic system. The GABAergic system plays a crucial role in regulating neuronal excitability, and thus Gabra4 is important for normal neural function [2]. Genetic models, such as knockout mice, are valuable tools for studying Gabra4's functions.
In Gabra4 knockout mice, autistic-like behavior, enhanced spatial memory, and attenuated susceptibility to pentylenetetrazol-induced seizures were observed, resembling human high-functioning autism. Hippocampal transcriptome profiling and interactome network construction revealed an up-regulation of the NMDAR system at the center of converged pathways underlying these phenotypes [1].
In conclusion, Gabra4 is essential for normal neural function through its role in the GABAergic synaptic transmission. The study of Gabra4 knockout mice has provided insights into its role in autism-like phenotypes and epilepsy, suggesting that Gabra4 may be a key factor in understanding the molecular mechanisms underlying these neurodevelopmental disorders.
References：
1. Fan, Cuixia, Gao, Yue, Liang, Guanmei, Gao, Tian-Ming, Yang, Xinping. 2020. Transcriptomics of Gabra4 knockout mice reveals common NMDAR pathways underlying autism, memory, and epilepsy. In Molecular autism, 11, 13. doi:10.1186/s13229-020-0318-9. https://pubmed.ncbi.nlm.nih.gov/32033586/
2. Bekdash, Rola A, Harrison, Neil L. 2015. Downregulation of Gabra4 expression during alcohol withdrawal is mediated by specific microRNAs in cultured mouse cortical neurons. In Brain and behavior, 5, e00355. doi:10.1002/brb3.355. https://pubmed.ncbi.nlm.nih.gov/26357588/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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