C57BL/6JCya-Grin2bem1flox/Cya
Common Name:
Grin2b-flox
Product ID:
S-CKO-02756
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Grin2b-flox
Strain ID
CKOCMP-14812-Grin2b-B6J-VA
Gene Name
Product ID
S-CKO-02756
Gene Alias
GluN2B; GluRepsilon2; NR2B; Nmdar2b
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Grin2bem1flox/Cya mice (Catalog S-CKO-02756) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000111905
NCBI RefSeq
NM_008171
Target Region
Exon 5
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
Grin2b, encoding the GluN2B subunit, is a gene crucial for normal neuronal development, learning, and memory. It is part of the N-methyl-D-aspartate receptor (NMDAR) gene family, with NMDARs being vital for synaptic transmission, plasticity, and neural circuit development [1,2].
In Grin2b-mutant mice with a patient-derived C456Y mutation (Grin2bC456Y/+), behavioral sensory hypersensitivity and brain hyperconnectivity associated with the anterior cingulate cortex (ACC) are observed. The ACC shows hyperactivity and hyperconnectivity, and pyramidal neurons in the ACC have increased excitatory synaptic transmission. Chemogenetic inhibition of ACC pyramidal neurons normalizes these phenotypes, suggesting GluN2B critically regulates ASD-related cortical connectivity and sensory brain functions [3].
In conclusion, Grin2b is essential for neuronal development, learning, and memory through its role in NMDARs. The study of Grin2b-mutant mice has provided insights into its role in sensory hypersensitivity and cortical connectivity, which may be relevant to understanding autism spectrum disorders.
References：
1. Korinek, M, Candelas Serra, M, Abdel Rahman, Fes, Balik, A, Smejkalova, T. 2024. Disease-Associated Variants in GRIN1, GRIN2A and GRIN2B genes: Insights into NMDA Receptor Structure, Function, and Pathophysiology. In Physiological research, 73, S413-S434. doi:. https://pubmed.ncbi.nlm.nih.gov/38836461/
2. Hu, Chun, Chen, Wenjuan, Myers, Scott J, Yuan, Hongjie, Traynelis, Stephen F. 2016. Human GRIN2B variants in neurodevelopmental disorders. In Journal of pharmacological sciences, 132, 115-121. doi:10.1016/j.jphs.2016.10.002. https://pubmed.ncbi.nlm.nih.gov/27818011/
3. Lee, Soowon, Jung, Won Beom, Moon, Heera, Kim, Seong-Gi, Kim, Eunjoon. 2024. Anterior cingulate cortex-related functional hyperconnectivity underlies sensory hypersensitivity in Grin2b-mutant mice. In Molecular psychiatry, 29, 3195-3207. doi:10.1038/s41380-024-02572-y. https://pubmed.ncbi.nlm.nih.gov/38704508/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen