C57BL/6JCya-Il17aem1flox/Cya
Common Name:
Il17a-flox
Product ID:
S-CKO-03087
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Il17a-flox
Strain ID
CKOCMP-16171-Il17a-B6J-VA
Gene Name
Product ID
S-CKO-03087
Gene Alias
Ctla-8; Ctla8; IL-17; IL-17A; Il17
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Il17aem1flox/Cya mice (Catalog S-CKO-03087) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027061
NCBI RefSeq
NM_010552
Target Region
Exon 2
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Il17a, a major member of the IL-17 cytokine family, is mainly produced by T helper 17 (Th17) cells, with other cell types like CD8+ T cells also capable of its production. It has a host-protective capacity in healthy individuals but excessive elevation is linked to autoimmune diseases and cancer, playing a role in immune-related pathways [3].
In a COPD mouse model, selective knockout of Il17a in the airway epithelium significantly reduced neutrophilic infiltration, peribronchial inflammation, pro-inflammatory mediators, and mucus production. This shows that Il17a, expressed in the airway epithelium, can act in an autocrine manner to amplify inflammation and increase mucus production in COPD pathogenesis [1].
In HLA-B27 transgenic rats, a peptide-based vaccine targeting Il17a alleviated the spondyloarthritis phenotype, inhibiting enthesitis, bone destruction, and new bone formation [2].
In conclusion, Il17a is crucial in regulating immune-related processes. The gene knockout mouse models in COPD and spondyloarthritis research have revealed its role in promoting inflammation and disease-related phenotypes, highlighting its potential as a therapeutic target in these diseases.
References：
1. Wu, Mindan, Lai, Tianwen, Jing, Du, Wu, Xiaohong, Shen, Huahao. . Epithelium-derived IL17A Promotes Cigarette Smoke-induced Inflammation and Mucus Hyperproduction. In American journal of respiratory cell and molecular biology, 65, 581-592. doi:10.1165/rcmb.2020-0424OC. https://pubmed.ncbi.nlm.nih.gov/34186014/
2. Hayashi, Hiroki, Sun, Jiao, Yanagida, Yuka, Tomita, Tetsuya, Nakagami, Hironori. . Peptide-based vaccine targeting IL17A attenuates experimental spondyloarthritis in HLA-B27 transgenic rats. In RMD open, 9, . doi:10.1136/rmdopen-2022-002851. https://pubmed.ncbi.nlm.nih.gov/36737108/
3. Song, Meiying, Liang, Jie, Wang, Luoyang, Zhang, Li, Zhang, Bei. 2023. IL-17A functions and the therapeutic use of IL-17A and IL-17RA targeted antibodies for cancer treatment. In International immunopharmacology, 123, 110757. doi:10.1016/j.intimp.2023.110757. https://pubmed.ncbi.nlm.nih.gov/37579542/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen