C57BL/6JCya-Il1bem1flox/Cya
Common Name:
Il1b-flox
Product ID:
S-CKO-03092
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Il1b-flox
Strain ID
CKOCMP-16176-Il1b-B6J-VA
Gene Name
Product ID
S-CKO-03092
Gene Alias
IL-1beta; Il-1b
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Il1bem1flox/Cya mice (Catalog S-CKO-03092) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000028881
NCBI RefSeq
NM_008361
Target Region
Exon 4~5
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Il1b, encoding interleukin-1β, is a key cytokine in the inflammatory response pathway. It is crucial for the host-response to pathogens, but also contributes to damage in chronic diseases and acute tissue injury. IL-1β does not follow the conventional ER-Golgi secretion route, and its release mechanism is still being explored [2].
Single-cell profiling has identified IL1Bhi macrophages associated with inflammation in PD-1 inhibitor-induced inflammatory arthritis. These macrophages have enhanced NLRP3 inflammasome activity and communicate with exhausted CD8+ T cells, suggesting a different cellular and molecular pathway compared to rheumatoid arthritis and highlighting IL1Bhi macrophages as a potential therapeutic target [1]. In addition, certain IL1B gene polymorphisms are associated with diseases such as keratoconus, essential tremor, and may play a role in miscarriage due to BaP/BPDE exposure [3,4,5].
In conclusion, Il1b is essential for the inflammatory response, and its study through genetic models, especially in relation to various diseases like PD-1 inhibitor-induced arthritis, keratoconus, essential tremor, and miscarriage, has provided insights into disease mechanisms. Understanding Il1b function can potentially lead to new therapeutic strategies for these conditions.
References：
1. Zhou, Ziyue, Zhou, Xiaoxiang, Jiang, Xu, Yang, Huaxia, Zhang, Xuan. 2024. Single-cell profiling identifies IL1Bhi macrophages associated with inflammation in PD-1 inhibitor-induced inflammatory arthritis. In Nature communications, 15, 2107. doi:10.1038/s41467-024-46195-x. https://pubmed.ncbi.nlm.nih.gov/38453911/
2. Lopez-Castejon, Gloria, Brough, David. 2011. Understanding the mechanism of IL-1β secretion. In Cytokine & growth factor reviews, 22, 189-95. doi:10.1016/j.cytogfr.2011.10.001. https://pubmed.ncbi.nlm.nih.gov/22019906/
3. Harati-Sadegh, Mahdiyeh, Sargazi, Saman, Khorasani, Milad, Sheervalilou, Roghayeh, Saravani, Ramin. 2021. IL1A and IL1B gene polymorphisms and keratoconus susceptibility: evidence from an updated meta-analysis. In Ophthalmic genetics, 42, 503-513. doi:10.1080/13816810.2021.1925926. https://pubmed.ncbi.nlm.nih.gov/33978542/
4. Chen, Jie, Huang, Pei, He, Yachao, Chen, Shengdi, Ma, Jianfang. 2019. IL1B polymorphism is associated with essential tremor in Chinese population. In BMC neurology, 19, 99. doi:10.1186/s12883-019-1331-5. https://pubmed.ncbi.nlm.nih.gov/31092216/
5. Guo, Jiarong, Zhao, Jingsong, Tian, Peng, Yang, Yang, Zhang, Huidong. 2024. BaP/BPDE exposure causes human trophoblast cell dysfunctions and induces miscarriage by up-regulating lnc-HZ06-regulated IL1B. In Journal of hazardous materials, 476, 134741. doi:10.1016/j.jhazmat.2024.134741. https://pubmed.ncbi.nlm.nih.gov/38991640/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen