C57BL/6JCya-Il2em1flox/Cya
Common Name:
Il2-flox
Product ID:
S-CKO-03099
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Il2-flox
Strain ID
CKOCMP-16183-Il2-B6J-VA
Gene Name
Product ID
S-CKO-03099
Gene Alias
Il-2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Il2em1flox/Cya mice (Catalog S-CKO-03099) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029275
NCBI RefSeq
NM_008366
Target Region
Exon 3~4
Size of Effective Region
~3.9 kb
Detailed Document
Overview of Gene Research
Interleukin-2 (IL-2), a cytokine, is crucial for immune homeostasis. It impacts regulatory T (Treg) cells and fine-tunes effector lymphocyte responses [1]. IL-2 was first identified as a growth factor driving the expansion of activated human T cell populations, and its expression, receptor signaling, and biological actions have been extensively studied over the past 40 years [2]. It is involved in regulating CD4+ T cell differentiation and function, as well as the effector and memory responses of CD8+ T cells [2].
In a mouse model of chikungunya virus-induced arthritis, treatment with an IL-2/anti-IL-2 complex increased both Tregs and effector (Teff) T cells, without significantly reducing inflammation or disease scores, while the anti-IL-2 mAb alone, which moderately increased IL-2 levels and activated Tregs, decreased the average disease score. This indicates complex immune-modulating effects of IL-2 in this disease context [3,4]. Mathematical models have also been developed to study the impact of IL-2 modulation therapies on T cell dynamics, simulating the effects of different treatments such as injection of IL-2, anti-IL-2 antibodies, IL-2/anti-IL-2 immune-complexes, and mutant variants of IL-2 to understand the dose and timing conditions for promoting either immunity or tolerance [5].
In conclusion, IL-2 is a key cytokine in immune regulation, with its actions affecting T cell differentiation, function, and immune responses. Mouse models, such as those for chikungunya-induced arthritis, and mathematical models have provided insights into its role in disease and potential therapeutic applications. These studies help in understanding how to manipulate IL-2's actions for treating autoimmune diseases and cancer [1,2,3,4,5].
References:
1. Arenas-Ramirez, Natalia, Woytschak, Janine, Boyman, Onur. 2015. Interleukin-2: Biology, Design and Application. In Trends in immunology, 36, 763-777. doi:10.1016/j.it.2015.10.003. https://pubmed.ncbi.nlm.nih.gov/26572555/
2. Spolski, Rosanne, Li, Peng, Leonard, Warren J. . Biology and regulation of IL-2: from molecular mechanisms to human therapy. In Nature reviews. Immunology, 18, 648-659. doi:10.1038/s41577-018-0046-y. https://pubmed.ncbi.nlm.nih.gov/30089912/
3. Tritsch, Sarah R, Porzucek, Abigail J, Schwartz, Arnold M, Mores, Christopher N, Chang, Aileen Y. 2023. Effects of IL2/anti-IL2 antibody complex on chikungunya virus-induced arthritis in a mouse model. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.01.30.526329. https://pubmed.ncbi.nlm.nih.gov/36778306/
4. Tritsch, Sarah R, Porzucek, Abigail J, Schwartz, Arnold M, Mores, Christopher N, Chang, Aileen Y. 2023. Effects of rIL2/anti-IL2 antibody complex on chikungunya virus-induced chronic arthritis in a mouse model. In Scientific reports, 13, 7307. doi:10.1038/s41598-023-34578-x. https://pubmed.ncbi.nlm.nih.gov/37147383/
5. León, Kalet, García-Martínez, Karina, Carmenate, Tania. 2013. Mathematical Models of the Impact of IL2 Modulation Therapies on T Cell Dynamics. In Frontiers in immunology, 4, 439. doi:10.3389/fimmu.2013.00439. https://pubmed.ncbi.nlm.nih.gov/24376444/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen