C57BL/6JCya-Itgamem1flox/Cya
Common Name:
Itgam-flox
Product ID:
S-CKO-03158
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Itgam-flox
Strain ID
CKOCMP-16409-Itgam-B6J-VA
Gene Name
Product ID
S-CKO-03158
Gene Alias
CD11b/CD18; CR3; CR3A; Cd11b; F730045J24Rik; Ly-40; MAC1; Mac-1; Mac-1a
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Itgamem1flox/Cya mice (Catalog S-CKO-03158) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000120355
NCBI RefSeq
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Target Region
Exon 3
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Itgam, also known as Integrin subunit alpha M, encodes the CD11b chain of the Mac-1 integrin. Mac-1 integrin is involved in mediating cell adhesion of monocytes, macrophages, and granulocytes, as well as promoting phagocytosis of complement-coated particles. It is associated with immune and inflammatory response-related pathways and is of great biological importance in maintaining normal immune function [7].
In ischemic stroke, Itgam was identified as a critical gene. Quantitative assessment showed significant overexpression in patients. Its modulation influenced the secretion of inflammatory cytokines like IL-1β, IL-6, and TNF-α, and had an impact on neuronal apoptosis, suggesting it may be a potential therapeutic target [1].
In systemic lupus erythematosus (SLE), multiple meta-analyses demonstrated a significant association between ITGAM gene polymorphism (such as rs1143679) and SLE in multiple ethnic populations. The risk allele was related to increased serum calcification propensity in SLE patients, indicating an increased cardiovascular risk [2,5,6].
In neutrophilic asthma, Itgam was identified as a critical target to distinguish it from healthy/non-NA individuals, with a strong correlation between Itgam and macrophages [3].
In diabetic nephropathy and atherosclerosis, Itgam was found to be a key gene involved in basement membrane alterations, mainly expressed within macrophages, and therapeutic strategies targeting Itgam may mitigate basement membrane lesions [4].
In Kawasaki disease, NFIL3 aggravated human coronary artery endothelial cell injury by promoting Itgam transcription [8].
In conclusion, Itgam plays essential roles in immune and inflammatory responses. Model-based research, especially studies related to gene polymorphisms, has revealed its significance in diseases such as ischemic stroke, SLE, neutrophilic asthma, diabetic nephropathy, atherosclerosis, and Kawasaki disease. Understanding Itgam's functions provides potential directions for developing therapeutic strategies for these diseases.
References：
1. Hou, Lei, Li, Zhongchen, Guo, Xiaoli, Zhang, Liyong, Li, Zefu. 2024. ITGAM is a critical gene in ischemic stroke. In Aging, 16, 6852-6867. doi:10.18632/aging.205729. https://pubmed.ncbi.nlm.nih.gov/38637126/
2. Fan, Y, Li, L-H, Pan, H-F, Sun, Z-Q, Ye, D-Q. . Association of ITGAM polymorphism with systemic lupus erythematosus: a meta-analysis. In Journal of the European Academy of Dermatology and Venereology : JEADV, 25, 271-5. doi:10.1111/j.1468-3083.2010.03776.x. https://pubmed.ncbi.nlm.nih.gov/20629846/
3. Yan, Qian, Liu, Zixing, Chen, Yujing, Zhan, Shaofeng, Huang, Xiufang. 2023. ITGAM-macrophage modulation as a potential strategy for treating neutrophilic Asthma: insights from bioinformatics analysis and in vivo experiments. In Apoptosis : an international journal on programmed cell death, 29, 393-411. doi:10.1007/s10495-023-01914-5. https://pubmed.ncbi.nlm.nih.gov/37950848/
4. Lou, Yude, Li, Peng Hui, Liu, Xiao Qi, Chen, Chen Chen, Ma, Kun Ling. 2024. ITGAM-mediated macrophages contribute to basement membrane damage in diabetic nephropathy and atherosclerosis. In BMC nephrology, 25, 72. doi:10.1186/s12882-024-03505-1. https://pubmed.ncbi.nlm.nih.gov/38413872/
5. Halfon, Matthieu, Zhang, Li, Ehirchiou, Driss, Ribi, Camillo, Busso, Nathalie. 2023. ITGAM rs1143679 Variant in Systemic Lupus Erythematosus Is Associated with Increased Serum Calcification Propensity. In Genes, 14, . doi:10.3390/genes14051105. https://pubmed.ncbi.nlm.nih.gov/37239465/
6. Lee, Young Ho, Bae, Sang-Cheol. 2014. Association between the functional ITGAM rs1143679 G/A polymorphism and systemic lupus erythematosus/lupus nephritis or rheumatoid arthritis: an update meta-analysis. In Rheumatology international, 35, 815-23. doi:10.1007/s00296-014-3156-2. https://pubmed.ncbi.nlm.nih.gov/25315704/
7. Fagerholm, S C, MacPherson, M, James, M J, Sevier-Guy, C, Lau, C S. . The CD11b-integrin (ITGAM) and systemic lupus erythematosus. In Lupus, 22, 657-63. doi:10.1177/0961203313491851. https://pubmed.ncbi.nlm.nih.gov/23753600/
8. Chen, Li, Shangguan, Zhiyang, Dong, Zeya, Ding, Yunyun, Yang, Shulong. 2023. NFIL3 aggravates human coronary artery endothelial cell injury by promoting ITGAM transcription in Kawasaki disease. In Hematology (Amsterdam, Netherlands), 28, 2277502. doi:10.1080/16078454.2023.2277502. https://pubmed.ncbi.nlm.nih.gov/37933872/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen