C57BL/6JCya-Ldhaem1flox/Cya
Common Name:
Ldha-flox
Product ID:
S-CKO-03369
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ldha-flox
Strain ID
CKOCMP-16828-Ldha-B6J-VA
Gene Name
Product ID
S-CKO-03369
Gene Alias
Ldh1; Ldhm; l7R2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ldhaem1flox/Cya mice (Catalog S-CKO-03369) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000209984
NCBI RefSeq
NM_001136069
Target Region
Exon 3
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Lactate dehydrogenase A (LDHA), also known as LDH5, is a crucial enzyme in aerobic glycolysis. It catalyzes the final step of glycolysis, converting pyruvate to lactate. This process is significant as most cancer cells rely on enhanced tumor glycolysis to meet their energy demands, with LDHA playing a key role in this metabolic reprogramming [1]. Additionally, it is involved in lactate shuttle and symbiosis in cancer cells, which impacts prognosis.
CM-specific LDHA knockout mice showed inhibited cardiomyocyte proliferation, leading to worse cardiac function and lower survival rate in the neonatal apical resection model. In contrast, CM-specific overexpression of LDHA promoted cardiomyocyte proliferation and cardiac repair post-MI, indicating its role in cardiac repair [2]. MK/platelet-specific LdhA knockout mice had an increased number of platelets with accelerated MK maturation and proplatelet formation, suggesting a non-classical function of LDHA in translation that could be a target for thrombocytopenia therapy [3]. In human trophoblast cells, LDHA knockdown induced G0/G1 phase arrest and increased apoptosis, while overexpression reversed these effects, revealing a new regulatory pathway for LDHA/PI3K/AKT/FOXO1/CyclinD1 in trophoblast cell cycle and proliferation [4].
In conclusion, LDHA is essential for metabolic processes, especially in glycolysis. Gene knockout models in mice have been instrumental in revealing its role in various biological processes and disease conditions. In cardiac repair, platelet production, and trophoblast function, LDHA plays significant roles, providing potential therapeutic targets for related diseases.
References:
1. Sharma, Dolly, Singh, Mamta, Rani, Reshma. 2022. Role of LDH in tumor glycolysis: Regulation of LDHA by small molecules for cancer therapeutics. In Seminars in cancer biology, 87, 184-195. doi:10.1016/j.semcancer.2022.11.007. https://pubmed.ncbi.nlm.nih.gov/36371026/
2. Chen, Yijin, Wu, Guangkai, Li, Mengsha, Bin, Jianping, Chen, Yanmei. 2022. LDHA-mediated metabolic reprogramming promoted cardiomyocyte proliferation by alleviating ROS and inducing M2 macrophage polarization. In Redox biology, 56, 102446. doi:10.1016/j.redox.2022.102446. https://pubmed.ncbi.nlm.nih.gov/36057161/
3. Chen, Qidi, Xin, Min, Wang, Lingjun, Liu, Junling, Fan, Xuemei. . Inhibition of LDHA to induce eEF2 release enhances thrombocytopoiesis. In Blood, 139, 2958-2971. doi:10.1182/blood.2022015620. https://pubmed.ncbi.nlm.nih.gov/35176139/
4. Zhu, Yueyue, Wu, Fan, Hu, Jianing, Lin, Yi, Liu, Xiaorui. . LDHA deficiency inhibits trophoblast proliferation via the PI3K/AKT/FOXO1/CyclinD1 signaling pathway in unexplained recurrent spontaneous abortion. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 37, e22744. doi:10.1096/fj.202201219RR. https://pubmed.ncbi.nlm.nih.gov/36583693/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen