C57BL/6JCya-Lef1em1flox/Cya
Common Name:
Lef1-flox
Product ID:
S-CKO-03376
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Lef1-flox
Strain ID
CKOCMP-16842-Lef1-B6J-VA
Gene Name
Product ID
S-CKO-03376
Gene Alias
3000002B05; Lef-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lef1em1flox/Cya mice (Catalog S-CKO-03376) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029611
NCBI RefSeq
NM_010703
Target Region
Exon 7~8
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
Lef1, or lymphoid enhancer binding factor 1, is a nuclear mediator in the Wnt/β -catenin pathway, which is crucial for signal transduction from ectoderm to mesenchyme during the development of ectoderm-derived structures. It activates target gene expression in this pathway and is thus of great biological importance in various developmental and cellular processes [1].
Targeted inactivation of the Lef1 gene in mice results in a complete block of development of multiple ectodermal appendages, indicating its essential role in ectodermal development [1]. In a cartilage-specific Lef1 knockout (KO) mouse model for posttraumatic osteoarthritis (PTOA), Lef1 ablation alleviated cartilage mineralization, reduced OA severity and local pain, suggesting Lef1's role in cartilage mineralization and OA-related phenotypes [2].
In conclusion, Lef1 is vital for ectodermal development and plays a role in cartilage mineralization and OA. The Lef1 KO mouse models have been instrumental in revealing its functions in these disease-related biological processes, providing insights into potential therapeutic strategies for ectodermal dysplasia and OA.
References:
1. Lévy, Jonathan, Capri, Yline, Rachid, Myriam, Tabet, Anne-Claude, Bailleul-Forestier, Isabelle. 2020. LEF1 haploinsufficiency causes ectodermal dysplasia. In Clinical genetics, 97, 595-600. doi:10.1111/cge.13714. https://pubmed.ncbi.nlm.nih.gov/32022899/
2. Elayyan, Jinan, Carmon, Idan, Zecharyahu, Lital, Klutstein, Michael, Dvir-Ginzberg, Mona. 2022. Lef1 ablation alleviates cartilage mineralization following posttraumatic osteoarthritis induction. In Proceedings of the National Academy of Sciences of the United States of America, 119, e2116855119. doi:10.1073/pnas.2116855119. https://pubmed.ncbi.nlm.nih.gov/35594394/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen