C57BL/6JCya-Loxem1flox/Cya
Common Name:
Lox-flox
Product ID:
S-CKO-03418
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Lox-flox
Strain ID
CKOCMP-16948-Lox-B6J-VA
Gene Name
Product ID
S-CKO-03418
Gene Alias
TSC-160; rrg
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
18
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Loxem1flox/Cya mice (Catalog S-CKO-03418) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025409
NCBI RefSeq
NM_010728
Target Region
Exon 1~2
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Lox, short for Lysyl Oxidase, is a Cu-dependent enzyme. It plays a primary catalytic role in the assembly of the extracellular matrix (ECM), which is crucial for cell fate, differentiation, and communication during development, tissue maintenance, and repair [2]. It is involved in multiple signaling pathways, such as those related to EGFR, PDGF, VEGF, TGF-β, mechano-transduction, inflammatory, and steroid signaling [2].
In animal models, LOX-1 knockout mice exhibit reduced intima thickness and inflammation, along with increased expression of protective factors, indicating its key role in atherogenic processes [1]. In hypoxic-ischemic brain damage, elevated LOX expression correlates with the severity of hypoxic-ischemic encephalopathy (HIE). Pharmacological inhibition of LOX ameliorates brain neuronal ferroptosis and improves learning and memory impairments [3].
In conclusion, Lox is essential for ECM assembly and participates in various signaling pathways. Gene knockout models in mice have revealed its significance in atherosclerosis and hypoxic-ischemic brain damage, providing insights into potential therapeutic targets for these diseases.
References:
1. Pirillo, Angela, Norata, Giuseppe Danilo, Catapano, Alberico Luigi. 2013. LOX-1, OxLDL, and atherosclerosis. In Mediators of inflammation, 2013, 152786. doi:10.1155/2013/152786. https://pubmed.ncbi.nlm.nih.gov/23935243/
2. Laczko, Rozalia, Csiszar, Katalin. 2020. Lysyl Oxidase (LOX): Functional Contributions to Signaling Pathways. In Biomolecules, 10, . doi:10.3390/biom10081093. https://pubmed.ncbi.nlm.nih.gov/32708046/
3. Jiang, Dongya, Zhao, Jing, Zheng, Jie, Wang, Long, Dong, Xiaohua. 2024. LOX-mediated ECM mechanical stress induces Piezo1 activation in hypoxic-ischemic brain damage and identification of novel inhibitor of LOX. In Redox biology, 76, 103346. doi:10.1016/j.redox.2024.103346. https://pubmed.ncbi.nlm.nih.gov/39260063/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen