C57BL/6JCya-Mafkem1flox/Cya
Common Name:
Mafk-flox
Product ID:
S-CKO-03623
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mafk-flox
Strain ID
CKOCMP-17135-Mafk-B6J-VA
Gene Name
Product ID
S-CKO-03623
Gene Alias
NF-E2; Nfe2u
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mafkem1flox/Cya mice (Catalog S-CKO-03623) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000018287
NCBI RefSeq
NM_010757
Target Region
Exon 3
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
MafK, a member of the small Maf family of basic region leucine zipper (bZIP)-type transcription factors, forms homodimers or heterodimers with cap 'n' collar (CNC) proteins and Bach proteins [3]. It lacks a transcriptional activation domain, and its homodimers act as transcriptional repressors, while its heterodimers can be involved in transcriptional activation or repression depending on the partner [3]. MafK is associated with pathways like stress response, detoxification, and is linked to various diseases such as ulcerative colitis, diabetes, neuronal disorders, thrombocytopenia, and carcinogenesis [1,4].
MafK overexpressing transgenic mice were more susceptible to Salmonella mucosal infection, with higher mortality, weight loss, intestinal damage, and inflammation. MafK promoted epithelial cell apoptosis via caspase-3 activation, contributing to Salmonella dissemination and inflammation [1]. In non-immune cells, MafK mediates chromatin remodeling to silence IRF8 expression in a cell-type-specific manner [2]. β-cell-specific Mafk overexpression in transgenic mice impaired pancreatic endocrine cell development, with disorganized islets, reduced β-cell proliferation, and altered expression of β-cell-related genes [6]. Mafg -/-:Mafk -/- double knockout mice had lens defects during embryogenesis, with mis-expression of genes related to the cytoskeleton, cell cycle, extracellular matrix, and ephrin signaling [5].
In conclusion, MafK plays crucial roles in various biological processes such as pathogen infection response, gene silencing, and organ development. Mouse models, including overexpression and knockout models, have been instrumental in revealing its functions in diseases like Salmonella-induced mucosal infections, and in understanding its role in pancreatic and lens development.
References:
1. Xu, Shiyao, Hu, Guiqiu, Wu, Di, Liu, Juxiong, Zhang, Chuan. 2022. MafK accelerates Salmonella mucosal infection through caspase-3 activation. In Aging, 14, 2287-2303. doi:10.18632/aging.203938. https://pubmed.ncbi.nlm.nih.gov/35260530/
2. Fourier, Nitsan, Zolty, Maya, Azriel, Aviva, Tedesco, Donato, Levi, Ben-Zion. 2020. MafK Mediates Chromatin Remodeling to Silence IRF8 Expression in Non-immune Cells in a Cell Type-SpecificManner. In Journal of molecular biology, 432, 4544-4560. doi:10.1016/j.jmb.2020.06.005. https://pubmed.ncbi.nlm.nih.gov/32534063/
3. Katsuoka, Fumiki, Yamamoto, Masayuki. 2016. Small Maf proteins (MafF, MafG, MafK): History, structure and function. In Gene, 586, 197-205. doi:10.1016/j.gene.2016.03.058. https://pubmed.ncbi.nlm.nih.gov/27058431/
4. Kannan, Meenakshi B, Solovieva, Vera, Blank, Volker. 2012. The small MAF transcription factors MAFF, MAFG and MAFK: current knowledge and perspectives. In Biochimica et biophysica acta, 1823, 1841-6. doi:10.1016/j.bbamcr.2012.06.012. https://pubmed.ncbi.nlm.nih.gov/22721719/
5. Patel, Shaili D, Anand, Deepti, Motohashi, Hozumi, Yamamoto, Masayuki, Lachke, Salil A. 2022. Deficiency of the bZIP transcription factors Mafg and Mafk causes misexpression of genes in distinct pathways and results in lens embryonic developmental defects. In Frontiers in cell and developmental biology, 10, 981893. doi:10.3389/fcell.2022.981893. https://pubmed.ncbi.nlm.nih.gov/36092713/
6. Abdellatif, Ahmed M, Oishi, Hisashi, Itagaki, Takahiro, Sugiyama, Fumihiro, Takahashi, Satoru. 2016. β-Cell-Specific Mafk Overexpression Impairs Pancreatic Endocrine Cell Development. In PloS one, 11, e0150010. doi:10.1371/journal.pone.0150010. https://pubmed.ncbi.nlm.nih.gov/26901059/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen