C57BL/6JCya-Mgat1em1flox/Cya
Common Name:
Mgat1-flox
Product ID:
S-CKO-03724
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Mgat1-flox
Strain ID
CKOCMP-17308-Mgat1-B6J-VA
Gene Name
Product ID
S-CKO-03724
Gene Alias
Mgat-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mgat1em1flox/Cya mice (Catalog S-CKO-03724) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000167400
NCBI RefSeq
NM_001110148
Target Region
Exon 4
Size of Effective Region
~2.9 kb
Detailed Document
Overview of Gene Research
MGAT1, also known as UDP-GlcNAc:alpha3-D-mannoside beta1,2-N-acetylglucosaminyltransferase I (GnTI) or N-acetylglucosaminyltransferase I (GlcNAcT-1), is a key enzyme in N-glycosylation. It initiates the synthesis of complex N-glycans, which are involved in multiple biological processes, such as cell signaling, immune response, and protein stability. The Wnt/β-catenin signaling pathway can up-regulate MGAT1 at transcriptional and post-transcriptional levels [4].
Conditional deletion of the mouse Mgat1 gene (Mgat1 cKO) in spermatogonia causes a germ-cell autonomous defect leading to infertility. Loss of complex N-glycans due to Mgat1 deletion promotes premature up-regulation of genes in spermatogenesis and spermiogenesis, and affects ERK1/2 signaling, potentially via different mechanisms [3].
In pancreatic ductal adenocarcinoma (PDAC), high expression of MGAT1 is associated with better patient prognosis, and its overexpression inhibits PDAC cell proliferation and migration [1]. In Wilms' tumor, LINC00173 promotes tumor progression through MGAT1-mediated MUC3A N-glycosylation [2]. In hepatocellular carcinoma (HCC), MGAT1 is identified as a promising therapeutic target through single-cell and spatial transcriptomics analysis [5]. In triple-negative breast cancer, overexpression of MGAT1 leads to immune evasion by regulating CD73 membrane translocation [6]. In pancreatic cancer, microRNA-204-3p inhibits metastasis by down-regulating MGAT1 [7].
In conclusion, MGAT1 plays essential roles in multiple biological processes and diseases. Mgat1 cKO mouse models have revealed its significance in spermatogenesis and infertility. In various cancers like PDAC, Wilms' tumor, HCC, and triple-negative breast cancer, as well as in pancreatic cancer metastasis, MGAT1 is involved in regulating disease-related cellular behaviors. These findings provide important insights into the biological functions of MGAT1 and potential therapeutic targets for related diseases.
References:
1. Jiang, Lai, Liu, Jie, Zhang, Shengke, Chi, Hao, Li, Bo. 2024. Role of glycosylation-related gene MGAT1 in pancreatic ductal adenocarcinoma. In Frontiers in immunology, 15, 1438935. doi:10.3389/fimmu.2024.1438935. https://pubmed.ncbi.nlm.nih.gov/39156890/
2. Zhu, Qingliang, Zhan, Deming, Yang, Yongguo, Xue, Haoliang, Zhu, Peng. 2022. LINC00173 promotes Wilms' tumor progression through MGAT1-mediated MUC3A N-glycosylation. In Cell cycle (Georgetown, Tex.), 21, 1795-1810. doi:10.1080/15384101.2022.2070399. https://pubmed.ncbi.nlm.nih.gov/35491865/
3. Biswas, Barnali, Batista, Frank, Sundaram, Subha, Stanley, Pamela. 2018. MGAT1 and Complex N-Glycans Regulate ERK Signaling During Spermatogenesis. In Scientific reports, 8, 2022. doi:10.1038/s41598-018-20465-3. https://pubmed.ncbi.nlm.nih.gov/29386567/
4. Akiva, Izzet, Birgül Iyison, Necla. 2018. MGAT1 is a novel transcriptional target of Wnt/β-catenin signaling pathway. In BMC cancer, 18, 60. doi:10.1186/s12885-017-3960-7. https://pubmed.ncbi.nlm.nih.gov/29310626/
5. Li, Yang, Chen, Yuan, Wang, Danqiong, An, Na, Yang, Haikun. 2024. Elucidating the multifaceted role of MGAT1 in hepatocellular carcinoma: integrative single-cell and spatial transcriptomics reveal novel therapeutic insights. In Frontiers in immunology, 15, 1442722. doi:10.3389/fimmu.2024.1442722. https://pubmed.ncbi.nlm.nih.gov/39081317/
6. Chi, Junlong Jack, Xie, Ping, Cheng, Mary Hongying, Zhang, Bin, Wan, Yong. 2025. MGAT1-Guided complex N-Glycans on CD73 regulate immune evasion in triple-negative breast cancer. In Nature communications, 16, 3552. doi:10.1038/s41467-025-58524-9. https://pubmed.ncbi.nlm.nih.gov/40229283/
7. Liu, Wei, Li, Xinglei, Tan, Xiao, Huang, Xing, Tian, Bole. . MicroRNA-204-3p inhibits metastasis of pancreatic cancer via downregulating MGAT1. In Journal of B.U.ON. : official journal of the Balkan Union of Oncology, 26, 2149-2156. doi:. https://pubmed.ncbi.nlm.nih.gov/34761629/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen