C57BL/6JCya-Nedd8em1flox/Cya
Common Name
Nedd8-flox
Product ID
S-CKO-03918
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-18002-Nedd8-B6J-VA
When using this mouse strain in a publication, please cite “Nedd8-flox Mouse (Catalog S-CKO-03918) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Nedd8-flox
Strain ID
CKOCMP-18002-Nedd8-B6J-VA
Gene Name
Product ID
S-CKO-03918
Gene Alias
NEDD-8, Rub1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 14
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000010520
NCBI RefSeq
NM_008683
Target Region
Exon 2~3
Size of Effective Region
~1.4 kb
Overview of Gene Research
Nedd8, a ubiquitin-like protein, plays a crucial role in eukaryotic cell biology. It is involved in the regulation of cullin-RING E3 ligase (CRL)-catalysed protein ubiquitylation. Neddylation, the process of attaching Nedd8 to substrate proteins, is a key post-translational modification in diverse biological pathways [1,3].
Genetic deletion of Nedd8 in cancer cells, as shown in triple-negative breast cancer (TNBC) models, causes a vulnerability to nivolumab (anti-PD-1). Although initial cell division is delayed, cell proliferation recovers. Nedd8-deficient cells exhibit enhanced immunogenicity, and in female immunocompetent mice, PD-1 blockade leads to CD8+ T-cell-mediated tumor regression in Nedd8-deficient EO771 tumors [2].
In conclusion, Nedd8 is essential for regulating CRL-mediated protein ubiquitylation. Its loss in cancer cells, especially in TNBC, reveals a potential for synergistic treatment with immune checkpoint blockade therapies. Understanding Nedd8's function through gene-knockout models provides insights into its role in disease and potential therapeutic strategies.
References：
1. Baek, Kheewoong, Krist, David T, Prabu, J Rajan, Kleiger, Gary, Schulman, Brenda A. 2020. NEDD8 nucleates a multivalent cullin-RING-UBE2D ubiquitin ligation assembly. In Nature, 578, 461-466. doi:10.1038/s41586-020-2000-y. https://pubmed.ncbi.nlm.nih.gov/32051583/
2. Papakyriacou, Irineos, Kutkaite, Ginte, Rúbies Bedós, Marta, Menden, Michael P, Mao, Yumeng. 2024. Loss of NEDD8 in cancer cells causes vulnerability to immune checkpoint blockade in triple-negative breast cancer. In Nature communications, 15, 3581. doi:10.1038/s41467-024-47987-x. https://pubmed.ncbi.nlm.nih.gov/38678024/
3. Schwechheimer, Claus. 2018. NEDD8-its role in the regulation of Cullin-RING ligases. In Current opinion in plant biology, 45, 112-119. doi:10.1016/j.pbi.2018.05.017. https://pubmed.ncbi.nlm.nih.gov/29909289/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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