C57BL/6JCya-Notch1em1flox/Cya
Common Name:
Notch1-flox
Product ID:
S-CKO-03993
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Notch1-flox
Strain ID
CKOCMP-18128-Notch1-B6J-VA
Gene Name
Product ID
S-CKO-03993
Gene Alias
9930111A19Rik; Mis6; N1; Tan1; lin-12
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Notch1em1flox/Cya mice (Catalog S-CKO-03993) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000028288
NCBI RefSeq
NM_008714
Target Region
Exon 3~4
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
Notch1 is a gene encoding a single-pass transmembrane receptor. The Notch signaling pathway, in which Notch1 is a key component, is crucial for cell-fate determination during development, regulating processes like tissue homeostasis, differentiation, and proliferation [3,5].
In hematological malignancies, NOTCH1 activating mutations are identified in over 50% of T-cell acute lymphoblastic leukemias and 12% of chronic lymphocytic leukemias, highlighting its role in leukemia pathogenesis [2]. In ischemic heart disease, aberrant Notch1 signaling is universal, affecting endothelial cells, vascular smooth muscle cells, and macrophages, as well as various remodeling stages [1]. In gastric cancer, the Notch1/CDH5 axis promotes cancer cell growth and metastasis [4].
In conclusion, Notch1 plays essential roles in development and disease. Its study, especially through gene knockout or conditional knockout mouse models (implied by functional studies in the references), has revealed its significance in diseases such as leukemia, ischemic heart disease, and gastric cancer, providing potential therapeutic targets.
References：
1. Peng, Xiafeng, Wang, Shixin, Chen, Hongwu, Chen, Minglong. 2023. Role of the Notch1 signaling pathway in ischemic heart disease (Review). In International journal of molecular medicine, 51, . doi:10.3892/ijmm.2023.5230. https://pubmed.ncbi.nlm.nih.gov/36799152/
2. Roti, Giovanni, Stegmaier, Kimberly. . Targeting NOTCH1 in hematopoietic malignancy. In Critical reviews in oncogenesis, 16, 103-15. doi:. https://pubmed.ncbi.nlm.nih.gov/22150311/
3. Boerkoel, Pierre, Huynh, Stephanie, Yang, Gui Xiang, Terry, Jefferson, Elbert, Adrienne. 2023. NOTCH1 loss of the TAD and PEST domain: An antimorph? In American journal of medical genetics. Part A, 191, 1593-1598. doi:10.1002/ajmg.a.63167. https://pubmed.ncbi.nlm.nih.gov/36866832/
4. Zhou, Lingshan, Yang, Yuan, Ye, Yuwei, Liu, Min, Zhou, Yongning. 2024. Notch1 signaling pathway promotes growth and metastasis of gastric cancer via modulating CDH5. In Aging, 16, 11893-11903. doi:10.18632/aging.206061. https://pubmed.ncbi.nlm.nih.gov/39172098/
5. Padovano, Costanzo, Bianco, Salvatore Daniele, Sansico, Francesca, Mazza, Tommaso, Giambra, Vincenzo. 2023. The Notch1 signaling pathway directly modulates the human RANKL-induced osteoclastogenesis. In Scientific reports, 13, 21199. doi:10.1038/s41598-023-48615-2. https://pubmed.ncbi.nlm.nih.gov/38040752/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen