C57BL/6JCya-Ogdhem1flox/Cya
Common Name:
Ogdh-flox
Product ID:
S-CKO-04070
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ogdh-flox
Strain ID
CKOCMP-18293-Ogdh-B6J-VA
Gene Name
Product ID
S-CKO-04070
Gene Alias
2210403E04Rik; 2210412K19Rik; E1o; OGDH-E1; d1401; mKIAA4192
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ogdhem1flox/Cya mice (Catalog S-CKO-04070) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000093350
NCBI RefSeq
NM_001252283
Target Region
Exon 6
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Ogdh, also known as oxoglutarate dehydrogenase, is the first rate-limiting E1 subunit of the OGDH complex (OGDHC). It is part of the tricarboxylic acid (TCA) cycle, catalyzing α-ketoglutarate (α-KG) to succinyl-CoA, playing a crucial role in glycometabolism. The TCA cycle is fundamental for energy production and also impacts various cellular processes [1,2,3].
In glioblastoma, loss of OGDH function by the drug CPI-613 was synthetically lethal with Bcl-xL inhibition in patient-derived xenografts and neurosphere GBM cultures, suggesting a potential treatment strategy [1]. Biallelic variants in OGDH cause a neurodevelopmental disorder with metabolic and movement abnormalities [2]. In classical swine fever virus infection, CSFV reduces OGDH protein levels, and OGDH overexpression inhibits CSFV proliferation while knockdown increases it, with OGDH regulating the AMPK-mTOR-autophagy pathway [3]. In gastric cancer, OGDH promotes cancer progression by enhancing mitochondrial function and activating the Wnt/β-catenin signaling pathway, and SIRT5 suppresses GC cell growth through desuccinylating OGDH [4,5]. In sepsis-induced acute lung injury, OGDH is highly expressed, and its down-regulation alleviates the injury, with OGDH acting through the MAPK pathway to release pro-inflammatory factors [6].
In conclusion, Ogdh is essential for the TCA cycle and glycometabolism. Studies using gene-related models have revealed its significant roles in various disease conditions such as glioblastoma, neurodevelopmental disorders, viral infections, gastric cancer, and sepsis-induced acute lung injury. Understanding Ogdh's functions provides potential targets for treating these diseases.
References:
1. Nguyen, Trang Tt, Torrini, Consuelo, Shang, Enyuan, Canoll, Peter, Siegelin, Markus D. 2024. OGDH and Bcl-xL loss causes synthetic lethality in glioblastoma. In JCI insight, 9, . doi:10.1172/jci.insight.172565. https://pubmed.ncbi.nlm.nih.gov/38483541/
2. Whittle, Ella F, Chilian, Madison, Karimiani, Ehsan Ghayoor, Yoon, Wan Hee, Carroll, Christopher J. 2022. Biallelic variants in OGDH encoding oxoglutarate dehydrogenase lead to a neurodevelopmental disorder characterized by global developmental delay, movement disorder, and metabolic abnormalities. In Genetics in medicine : official journal of the American College of Medical Genetics, 25, 100332. doi:10.1016/j.gim.2022.11.001. https://pubmed.ncbi.nlm.nih.gov/36520152/
3. Zeng, Sen, Zhu, Wenhui, Luo, Zipeng, Fan, Shuangqi, Chen, Jinding. 2023. Role of OGDH in Atophagy-IRF3-IFN-β pathway during classical swine fever virus infection. In International journal of biological macromolecules, 249, 126443. doi:10.1016/j.ijbiomac.2023.126443. https://pubmed.ncbi.nlm.nih.gov/37604413/
4. Lu, Xin, Wu, Nan, Yang, Wanli, Yan, Kemin, Wu, Jing. 2019. OGDH promotes the progression of gastric cancer by regulating mitochondrial bioenergetics and Wnt/β-catenin signal pathway. In OncoTargets and therapy, 12, 7489-7500. doi:10.2147/OTT.S208848. https://pubmed.ncbi.nlm.nih.gov/31686854/
5. Lu, Xin, Yang, Pengfei, Zhao, Xinrui, Zeng, Li, Wu, Jing. 2019. OGDH mediates the inhibition of SIRT5 on cell proliferation and migration of gastric cancer. In Experimental cell research, 382, 111483. doi:10.1016/j.yexcr.2019.06.028. https://pubmed.ncbi.nlm.nih.gov/31247190/
6. Hao, Yuewei, Wang, Zheng, Wang, Xinfang, Zhan, Wenming, Wu, Dianshui. . OGDH is involved in sepsis induced acute lung injury through the MAPK pathway. In Journal of thoracic disease, 13, 5042-5054. doi:10.21037/jtd-21-948. https://pubmed.ncbi.nlm.nih.gov/34527342/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen