C57BL/6JCya-Or6a2em1flox/Cya
Common Name:
Or6a2-flox
Product ID:
S-CKO-04083
Background:
C57BL/6JCya
Product Type
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Genotype
Sex
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Basic Information
Strain Name
Or6a2-flox
Strain ID
CKOCMP-18317-Or6a2-B6J-VA
Gene Name
Product ID
S-CKO-04083
Gene Alias
I7; MOR103-15; Olfr2; Olfr41
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Or6a2em1flox/Cya mice (Catalog S-CKO-04083) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000094109
NCBI RefSeq
NM_010983
Target Region
Exon 2
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
OR6A2, the human ortholog of mouse Olfr2, is an olfactory receptor belonging to the G protein–coupled chemoreceptor family. It plays a role in detecting the compound octanal, which is involved in the activation of the NLRP3 inflammasome and induction of interleukin-1β secretion in macrophages. This activation is associated with the inflammatory response, and is relevant to the development of atherosclerosis [1,2,3].
Genetic targeting of Olfr2 in mice (a form of gene knockout model) significantly reduced atherosclerotic plaques. Boosting octanal levels exacerbated atherosclerosis, indicating that the activation of Olfr2 (and by extension OR6A2) through octanal binding drives the progression of atherosclerosis [1].
In conclusion, OR6A2 is involved in the NLRP3-dependent IL-1 production pathway in macrophages. The gene knockout models of its mouse ortholog Olfr2 have revealed its crucial role in the development of atherosclerosis, suggesting that inhibiting OR6A2 may be a promising strategy for preventing and treating this disease [1].
References：
1. Orecchioni, Marco, Kobiyama, Kouji, Winkels, Holger, Hazen, Stanley L, Ley, Klaus. 2022. Olfactory receptor 2 in vascular macrophages drives atherosclerosis by NLRP3-dependent IL-1 production. In Science (New York, N.Y.), 375, 214-221. doi:10.1126/science.abg3067. https://pubmed.ncbi.nlm.nih.gov/35025664/
2. Orecchioni, Marco, Matsunami, Hiroaki, Ley, Klaus. 2022. Olfactory receptors in macrophages and inflammation. In Frontiers in immunology, 13, 1029244. doi:10.3389/fimmu.2022.1029244. https://pubmed.ncbi.nlm.nih.gov/36311776/
3. Wang, Congcong, Andreasson, Katrin I. 2022. Odorant receptors in macrophages: potential targets for atherosclerosis. In Trends in immunology, 43, 262-264. doi:10.1016/j.it.2022.02.006. https://pubmed.ncbi.nlm.nih.gov/35283015/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen