C57BL/6JCya-Pcnaem1flox/Cya
Common Name
Pcna-flox
Product ID
S-CKO-04184
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-18538-Pcna-B6J-VA
When using this mouse strain in a publication, please cite “Pcna-flox Mouse (Catalog S-CKO-04184) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Pcna-flox
Strain ID
CKOCMP-18538-Pcna-B6J-VA
Gene Name
Product ID
S-CKO-04184
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 2
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000028817
NCBI RefSeq
NM_011045
Target Region
Exon 2~4
Size of Effective Region
~2.2 kb
Overview of Gene Research
Pcna, short for Proliferating cell nuclear antigen, is an essential factor in DNA replication and repair pathways [1,3,4]. It forms a homotrimeric ring that encircles the DNA, acting as a processivity factor for DNA polymerases and a landing platform for proteins interacting with DNA and chromatin. As such, it is a key coordinator of essential cellular functions for cell growth, death, and maintenance [3,4].
In human cells, Pcna interacts with a wide variety of proteins through the PCNA-interacting protein (PIP) box, and understanding these interactions can potentially lead to the development of broad-spectrum cancer therapeutics [2]. For example, a small molecule Pcna inhibitor, AOH1996, selectively kills cancer cells by enhancing the interaction between Pcna and RPB1, dissociating Pcna from actively transcribed chromatin regions and inducing DNA double-stranded breaks in a transcription-dependent manner [5]. Also, ubiquitination of Pcna at lysine 164 is crucial for maintaining human genome stability as it promotes FANCD2-dependent mitotic DNA synthesis, preventing the accumulation of under-replicated DNA [6].
In conclusion, Pcna is vital for DNA replication and repair, and its interactions and post-translational modifications play key roles in maintaining genomic stability. The study of Pcna and its associated functions, as revealed through in vitro and in vivo studies, may offer potential therapeutic opportunities in cancer treatment [1,2,5].
References：
1. González-Magaña, Amaia, Blanco, Francisco J. 2020. Human PCNA Structure, Function and Interactions. In Biomolecules, 10, . doi:10.3390/biom10040570. https://pubmed.ncbi.nlm.nih.gov/32276417/
2. Horsfall, Aimee J, Abell, Andrew D, Bruning, John B. 2019. Targeting PCNA with Peptide Mimetics for Therapeutic Purposes. In Chembiochem : a European journal of chemical biology, 21, 442-450. doi:10.1002/cbic.201900275. https://pubmed.ncbi.nlm.nih.gov/31247123/
3. Arbel, Matan, Choudhary, Karan, Tfilin, Ofri, Kupiec, Martin. 2021. PCNA Loaders and Unloaders-One Ring That Rules Them All. In Genes, 12, . doi:10.3390/genes12111812. https://pubmed.ncbi.nlm.nih.gov/34828416/
4. Wang, Shao-Chun. 2014. PCNA: a silent housekeeper or a potential therapeutic target? In Trends in pharmacological sciences, 35, 178-86. doi:10.1016/j.tips.2014.02.004. https://pubmed.ncbi.nlm.nih.gov/24655521/
5. Gu, Long, Li, Min, Li, Caroline M, Perry, J Jefferson P, Malkas, Linda H. 2023. Small molecule targeting of transcription-replication conflict for selective chemotherapy. In Cell chemical biology, 30, 1235-1247.e6. doi:10.1016/j.chembiol.2023.07.001. https://pubmed.ncbi.nlm.nih.gov/37531956/
6. Leung, Wendy, Baxley, Ryan M, Traband, Emma, Shima, Naoko, Bielinsky, Anja-Katrin. 2023. FANCD2-dependent mitotic DNA synthesis relies on PCNA K164 ubiquitination. In Cell reports, 42, 113523. doi:10.1016/j.celrep.2023.113523. https://pubmed.ncbi.nlm.nih.gov/38060446/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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