C57BL/6JCya-Pou2af1em1flox/Cya
Common Name:
Pou2af1-flox
Product ID:
S-CKO-04382
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Pou2af1-flox
Strain ID
CKOCMP-18985-Pou2af1-B6J-VA
Gene Name
Product ID
S-CKO-04382
Gene Alias
BOB.1; Bob-1; Bob1; OBF-1; OBF.1; OCA-B; OCAB
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pou2af1em1flox/Cya mice (Catalog S-CKO-04382) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034554
NCBI RefSeq
NM_011136
Target Region
Exon 2~4
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
POU2AF1, also known as OCA-B, is a transcription co-factor. It plays crucial roles in various biological processes. It is involved in immune-related regulation, such as in the differentiation of Tfh cells where it promotes Tfh formation and migration into germinal centers by upregulating CXCR5 [2]. It also functions in the regulation of host defense genes in the human airway epithelium, helping maintain a host defense tone even in pathogen-free conditions [4].
In overweight patients, POU2AF1 expression was upregulated in retroperitoneal adipose tissue and increased during adipogenesis. Overexpression of POU2AF1 promoted MSCs adipogenesis, while its silencing inhibited adipogenesis, suggesting its role in adipose-related biological processes through the HDAC1 signaling pathway [1]. In B-cell malignancies dependent on POU2AF1, treatment with mSWI/SNF ATPase degraders led to chromatin eviction of POU2AF1 and decreased downstream signaling, indicating its importance in these malignancies [3].
In conclusion, POU2AF1 is a key transcription co-factor involved in diverse biological processes, including immune regulation, adipogenesis, and host defense. Its study, especially through functional studies in relevant disease models, provides insights into its role in diseases such as adiposity-related disorders and B-cell malignancies, which may offer potential therapeutic targets.
References：
1. Wang, Yaqing, Wang, Luyang, Su, Zhongping, Jiang, Li, Kong, Xiangqing. . POU2AF1 promotes MSCs adipogenesis by inhibiting HDAC1 expression. In Adipocyte, 10, 251-263. doi:10.1080/21623945.2021.1918863. https://pubmed.ncbi.nlm.nih.gov/33949290/
2. Zhu, Xiaoliang, Chen, Xi, Cao, Yaqiang, Zhao, Keji, Zhu, Jinfang. 2024. Optimal CXCR5 Expression during Tfh Maturation Involves the Bhlhe40-Pou2af1 Axis. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.05.16.594397. https://pubmed.ncbi.nlm.nih.gov/38903096/
3. He, Tongchen, Xiao, Lanbo, Qiao, Yuanyuan, Vakoc, Christopher R, Chinnaiyan, Arul M. 2024. Targeting the mSWI/SNF complex in POU2F-POU2AF transcription factor-driven malignancies. In Cancer cell, 42, 1336-1351.e9. doi:10.1016/j.ccell.2024.06.006. https://pubmed.ncbi.nlm.nih.gov/39029462/
4. Zhou, Haixia, Brekman, Angelika, Zuo, Wu-Lin, Crystal, Ronald G, Wang, Guoqing. 2016. POU2AF1 Functions in the Human Airway Epithelium To Regulate Expression of Host Defense Genes. In Journal of immunology (Baltimore, Md. : 1950), 196, 3159-67. doi:10.4049/jimmunol.1502400. https://pubmed.ncbi.nlm.nih.gov/26927796/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen