C57BL/6JCya-Ppp1caem1flox/Cya
Common Name:
Ppp1ca-flox
Product ID:
S-CKO-04411
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ppp1ca-flox
Strain ID
CKOCMP-19045-Ppp1ca-B6J-VA
Gene Name
Product ID
S-CKO-04411
Gene Alias
Ppp1c; dism2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ppp1caem1flox/Cya mice (Catalog S-CKO-04411) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000046094
NCBI RefSeq
NM_031868
Target Region
Exon 4~7
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Ppp1ca, encoding Protein phosphatase 1 catalytic subunit alpha, is a key component of the PP1 complex. It is involved in regulating diverse cellular processes such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription [7]. It participates in multiple pathways like the regulation of TFEB-dependent autophagy, glucose metabolism, and the AKT-mTOR signaling pathway, which are crucial for maintaining normal cellular functions and organismal health [1,2,3]. The generation of a PPP1CA knockout human pluripotent stem cell line using the Nuclease technology system provides a valuable research model for further exploring its functions [7].
In the context of diseases, in intervertebral disc degeneration, increased K141-methylation of PPP1CA disrupts its interaction with TFEB, blocking TFEB dephosphorylation and nuclear translocation, leading to autophagy deficiency and NPC senescence [1]. In osteoporosis, CARM1 methylates R23 of PPP1CA, affecting glucose metabolism-related phosphorylation events and ultimately influencing osteogenic and osteoclastic differentiation [2]. In clear cell renal cell carcinoma, a peptide encoded by circPDHK1 interacts with PPP1CA, inhibiting AKT dephosphorylation and activating the AKT-mTOR signaling pathway to promote cancer progression [3]. In diabetic retinopathy, PPP1CA activates retinal Müller cells through the PPP1CA/YAP/GS/Gln/mTORC1 pathway [4]. In colorectal cancer, circGPRC5A stabilizes PPP1CA protein, enhancing YAP dephosphorylation and promoting cancer progress [5]. In salt-sensitive hypertension, dietary potassium stimulates Ppp1Ca-Ppp1r1a-mediated dephosphorylation of the kidney NaCl cotransporter, reducing blood pressure [6]. In glioblastoma, KIF18A interacts with PPP1CA to promote the malignant development of the tumor [8].
In summary, Ppp1ca plays essential roles in multiple biological processes and is implicated in various disease conditions. The use of gene knockout models, such as the PPP1CA knockout human pluripotent stem cell line, has provided significant insights into its functions in different diseases, helping to understand the underlying mechanisms and potentially guiding the development of therapeutic strategies for these diseases.
References:
1. Liang, Huaizhen, Luo, Rongjin, Li, Gaocai, Song, Yu, Yang, Cao. 2023. Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration. In Cell death and differentiation, 30, 2135-2150. doi:10.1038/s41418-023-01210-4. https://pubmed.ncbi.nlm.nih.gov/37605006/
2. Zhang, Lu, Jiao, Guangjun, You, Yunhao, Meng, Chunyang, Chen, Yunzhen. . Arginine methylation of PPP1CA by CARM1 regulates glucose metabolism and affects osteogenic differentiation and osteoclastic differentiation. In Clinical and translational medicine, 13, e1369. doi:10.1002/ctm2.1369. https://pubmed.ncbi.nlm.nih.gov/37649137/
3. Huang, Bo, Ren, Junwu, Ma, Qiang, Wang, Yongquan, Xiao, Bin. 2024. A novel peptide PDHK1-241aa encoded by circPDHK1 promotes ccRCC progression via interacting with PPP1CA to inhibit AKT dephosphorylation and activate the AKT-mTOR signaling pathway. In Molecular cancer, 23, 34. doi:10.1186/s12943-024-01940-0. https://pubmed.ncbi.nlm.nih.gov/38360682/
4. Guo, Yang, Cang, Xiaomin, Zhu, Linling, Wang, Xueqin, Song, E. 2021. PPP1CA/YAP/GS/Gln/mTORC1 pathway activates retinal Müller cells during diabetic retinopathy. In Experimental eye research, 210, 108703. doi:10.1016/j.exer.2021.108703. https://pubmed.ncbi.nlm.nih.gov/34280391/
5. Chen, Zhenzhou, Li, Yidan, He, Kuan, Chen, Yajun, Fu, Zhongxue. 2023. CircGPRC5A enhances colorectal cancer progress by stabilizing PPP1CA and inducing YAP dephosphorylation. In Journal of experimental & clinical cancer research : CR, 42, 334. doi:10.1186/s13046-023-02915-7. https://pubmed.ncbi.nlm.nih.gov/38057879/
6. Grimm, P Richard, Tatomir, Anamaria, Rosenbaek, Lena L, Fenton, Robert A, Welling, Paul A. 2023. Dietary potassium stimulates Ppp1Ca-Ppp1r1a dephosphorylation of kidney NaCl cotransporter and reduces blood pressure. In The Journal of clinical investigation, 133, . doi:10.1172/JCI158498. https://pubmed.ncbi.nlm.nih.gov/37676724/
7. Wang, Xiaoman, Sun, Jiaan, Fu, Chong, Liu, Chang. 2023. Generation of a PPP1CA knockout human pluripotent stem cell line via Nuclease technology. In Stem cell research, 69, 103077. doi:10.1016/j.scr.2023.103077. https://pubmed.ncbi.nlm.nih.gov/36965407/
8. Yang, Ji, Zhang, Qiaorong, Yang, Ziyuan, Wang, Xiaolang, Liu, Xianxian. 2023. KIF18A interacts with PPP1CA to promote the malignant development of glioblastoma. In Experimental and therapeutic medicine, 25, 154. doi:10.3892/etm.2023.11853. https://pubmed.ncbi.nlm.nih.gov/36911368/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen