C57BL/6JCya-Hexim1em1flox/Cya
Common Name
Hexim1-flox
Product ID
S-CKO-04556
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-192231-Hexim1-B6J-VA
When using this mouse strain in a publication, please cite “Hexim1-flox Mouse (Catalog S-CKO-04556) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Hexim1-flox
Strain ID
CKOCMP-192231-Hexim1-B6J-VA
Gene Name
Product ID
S-CKO-04556
Gene Alias
Clp1, HIS1, CLP-1, 7330426E13Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 11
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000053063
NCBI RefSeq
NM_138753
Target Region
Exon 1
Size of Effective Region
~1.1 kb
Overview of Gene Research
Hexim1, also known as hexamethylene bis-acetamide inducible 1, is a protein that acts as a tumor suppressor and is involved in the regulation of innate immunity. It was initially described as a non-coding RNA-dependent regulator of transcription. Hexim1 binds to 7SK RNA and inhibits the positive transcription elongation factor (P-TEFb), thus playing a role in controlling transcription elongation. It is also involved in regulating the stability of transcription factor components and their binding to targets. Additionally, it participates in pathways related to erythropoiesis, cell cycle regulation, and hormone-related gene expression, with implications for diseases like cancer, AIDS, cardiac hypertrophy, and inflammation [1,3,4,5].
In human erythropoiesis, HEXIM1 is a key transcription regulator. Overexpression of HEXIM1 promotes erythroid proliferation and fetal globin expression. It regulates erythroid proliferation by influencing RNAPII pausing at cell cycle checkpoint genes and increasing RNAPII occupancy at genes promoting cycle progression. Genome-wide profiling shows changes in the distribution of GATA-binding factor 1 (GATA1) and RNAPII, especially at the β-globin loci, affecting fetal globin expression [2]. In alpha-herpesvirus (Anatid herpesvirus 1) infection, HEXIM1 is significantly upregulated. Excessive HEXIM1 assists the virus in progeny virus production, gene expression, and RNA polymerase II recruitment by promoting the formation of more inactive P-TEFb and loss of RNAPII S2 phosphorylation, while suppressing some host survival-related genes [6].
In conclusion, Hexim1 is an essential regulator in multiple biological processes. Its role in diseases such as those related to abnormal cell proliferation (cancer), viral infections (AIDS-related HIV transcription), and cardiac disorders (hypertrophy) is significant. Functional studies, especially those related to its role in transcription elongation and cell-cycle-related processes, contribute to our understanding of disease mechanisms. The insights from studies like those on its function in erythropoiesis and viral infections help in potentially developing therapeutic strategies for relevant diseases.
References：
1. Michels, Annemieke A, Bensaude, Olivier. 2018. Hexim1, an RNA-controlled protein hub. In Transcription, 9, 262-271. doi:10.1080/21541264.2018.1429836. https://pubmed.ncbi.nlm.nih.gov/29345523/
2. Lv, Xiurui, Murphy, Kristin, Murphy, Zachary, Mohandas, Narla, Steiner, Laurie A. . HEXIM1 is an essential transcription regulator during human erythropoiesis. In Blood, 142, 2198-2215. doi:10.1182/blood.2022019495. https://pubmed.ncbi.nlm.nih.gov/37738561/
3. Dey, Anwesha, Chao, Sheng-Hao, Lane, David P. 2007. HEXIM1 and the control of transcription elongation: from cancer and inflammation to AIDS and cardiac hypertrophy. In Cell cycle (Georgetown, Tex.), 6, 1856-63. doi:. https://pubmed.ncbi.nlm.nih.gov/17671421/
4. Chen, Ruichuan, Yik, Jasper H N, Lew, Qiao Jing, Chao, Sheng-Hao. 2014. Brd4 and HEXIM1: multiple roles in P-TEFb regulation and cancer. In BioMed research international, 2014, 232870. doi:10.1155/2014/232870. https://pubmed.ncbi.nlm.nih.gov/24592384/
5. Mozar, Fitya, Sharma, Vikas, Gorityala, Shashank, Xu, Yan, Montano, Monica M. . Downregulation of Dihydrotestosterone and Estradiol Levels by HEXIM1. In Endocrinology, 163, . doi:10.1210/endocr/bqab236. https://pubmed.ncbi.nlm.nih.gov/34864989/
6. Wu, Ying, Sun, Anyang, Yang, Qiqi, Wu, Zhen, Cheng, Anchun. 2024. An alpha-herpesvirus employs host HEXIM1 to promote viral transcription. In Journal of virology, 98, e0139223. doi:10.1128/jvi.01392-23. https://pubmed.ncbi.nlm.nih.gov/38363111/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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