C57BL/6JCya-Hsh2dem1flox/Cya
Common Name
Hsh2d-flox
Product ID
S-CKO-05461
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-209488-Hsh2d-B6J-VA
When using this mouse strain in a publication, please cite “Hsh2d-flox Mouse (Catalog S-CKO-05461) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Hsh2d-flox
Strain ID
CKOCMP-209488-Hsh2d-B6J-VA
Gene Name
Product ID
S-CKO-05461
Gene Alias
ALX, Hsh2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 8
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000072097
NCBI RefSeq
NM_197944
Target Region
Exon 4
Size of Effective Region
~0.8 kb
Overview of Gene Research
Hsh2d, short for haematopoietic SH2 domain containing, may play a role in multiple biological processes. It has been implicated in immune-related pathways as genes like Hsh2d have been associated with inflammatory/immune-related biological processes in lung macrophages [3]. Also, its expression was found to be related to interferon signaling-associated genes in doxorubicin-surviving proliferative cells [2].
In acute lymphoblastic leukaemia, Hsh2d was shown to contribute to methotrexate resistance in human T-cell acute lymphoblastic leukaemia. Downregulation of Hsh2d was observed in T-ALL compared with B-cell ALL. Overexpression of Hsh2d inhibited CD28-mediated IL-2 activation, and it was necessary for HuT-78 cells to be resistant to methotrexate [1].
In cervical squamous cell carcinoma, Hsh2d was part of an immune-based mRNA-lncRNA signature identified to predict overall survival [5].
In addition, in murine experimental colitis, the immune process-related factor Hsh2d was upregulated in colon tissues of macrophages with p38α deficiency, which ameliorated the colitis [4].
In summary, Hsh2d appears to have important functions in immune-related responses and disease resistance. Its role in conferring drug resistance in T-cell acute lymphoblastic leukaemia and its association with immune-based signatures in cervical squamous cell carcinoma highlight its significance in these disease areas. Also, its involvement in murine colitis through immune-related processes further emphasizes its importance in understanding disease mechanisms [1,4,5].
References：
1. Wang, Jing, Xiong, Yiying. 2020. HSH2D contributes to methotrexate resistance in human T‑cell acute lymphoblastic leukaemia. In Oncology reports, 44, 2121-2129. doi:10.3892/or.2020.7772. https://pubmed.ncbi.nlm.nih.gov/33000278/
2. Trzaskoma, Pawel, Jung, SeolKyoung, Kanno, Yuka, O'Shea, John J, Chow, Carson C. 2024. Doxorubicin resistance involves modulation of interferon signaling, transcriptional bursting, and gene co-expression patterns of U-ISGF3-related genes. In Neoplasia (New York, N.Y.), 58, 101071. doi:10.1016/j.neo.2024.101071. https://pubmed.ncbi.nlm.nih.gov/39405604/
3. Armstrong, David A, Chen, Youdinghuan, Dessaint, John A, Christensen, Brock C, Ashare, Alix. 2019. DNA Methylation Changes in Regional Lung Macrophages Are Associated with Metabolic Differences. In ImmunoHorizons, 3, 274-281. doi:10.4049/immunohorizons.1900042. https://pubmed.ncbi.nlm.nih.gov/31356157/
4. Chen, Wei, Liang, Rui, Yi, Youcai, Zhu, Jinshui, Zhang, Jing. 2022. P38α deficiency in macrophages ameliorates murine experimental colitis by regulating inflammation and immune process. In Pathology, research and practice, 233, 153881. doi:10.1016/j.prp.2022.153881. https://pubmed.ncbi.nlm.nih.gov/35430508/
5. Mao, Yifang, Chen, Run, Xia, Meng, Huang, Jiaming, He, Mian. 2021. Identification of an immune-based mRNA-lncRNA signature for overall survival in cervical squamous cell carcinoma. In Future oncology (London, England), 17, 2365-2380. doi:10.2217/fon-2020-1153. https://pubmed.ncbi.nlm.nih.gov/33724869/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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