C57BL/6JCya-Aldh4a1em1flox/Cya
Common Name:
Aldh4a1-flox
Product ID:
S-CKO-05652
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Aldh4a1-flox
Strain ID
CKOCMP-212647-Aldh4a1-B6J-VA
Gene Name
Product ID
S-CKO-05652
Gene Alias
A930035F14Rik; Ahd-1; Ahd1; Aldh4; Aldh5a1; E330022C09; P5cd; P5cdh; P5cdhl; P5cdhs; Ssdh1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Aldh4a1em1flox/Cya mice (Catalog S-CKO-05652) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000039818
NCBI RefSeq
NM_175438
Target Region
Exon 2~5
Size of Effective Region
~2.9 kb
Detailed Document
Overview of Gene Research
Aldh4a1, a member of the aldehyde dehydrogenase superfamily, is a key mitochondrial enzyme in the proline metabolism pathway. It catalyzes the final steps of both proline and hydroxyproline catabolism, converting L-glutamate-γ-semialdehyde to L-glutamate in proline metabolism and 4-hydroxy-L-glutamate-γ-semialdehyde to 4-erythro-hydroxy-L-glutamate in hydroxyproline metabolism [5,6,7].
A knockout mouse model of Aldh4a1 was constructed using CRISPR-Cas9 technology. Results showed that Aldh4a1^(-/-) male mice had normal spermatogenesis but defects in sperm maturation in the epididymis, with impaired motility, increased morphological abnormalities, and increased spontaneous acrosome reaction. Transmission electron microscopy revealed vacuoles in sperm mitochondria, fractures in sperm necks, and vacuoles, indicating that ALDH4A1 is vital for sperm flagellum structure and sperm maturation in mice [2].
In conclusion, Aldh4a1 plays a crucial role in proline and hydroxyproline catabolism and is essential for sperm maturation in mice. The Aldh4a1 knockout mouse model has provided valuable insights into its function in male reproduction. Additionally, ALDH4A1 has been implicated in other biological processes and diseases, such as atherosclerosis, schizophrenia, and muscle health, suggesting its broad-ranging importance in biology and medicine [1,3,4].
References：
1. Lorenzo, Cristina, Delgado, Pilar, Busse, Christian E, Wardemann, Hedda, Ramiro, Almudena R. 2020. ALDH4A1 is an atherosclerosis auto-antigen targeted by protective antibodies. In Nature, 589, 287-292. doi:10.1038/s41586-020-2993-2. https://pubmed.ncbi.nlm.nih.gov/33268892/
2. Xiao, Y, Wen, Z Z, Wu, B, Li, J Y, Gao, J G. . [Deletion of Aldh4a1 Leads to Impaired Sperm Maturation in Mice]. In Molekuliarnaia biologiia, 56, 585-594. doi:10.31857/S0026898422040152. https://pubmed.ncbi.nlm.nih.gov/35964315/
3. Nagaoka, Atsuko, Kunii, Yasuto, Hino, Mizuki, Kakita, Akiyoshi, Yabe, Hirooki. 2020. ALDH4A1 expression levels are elevated in postmortem brains of patients with schizophrenia and are associated with genetic variants in enzymes related to proline metabolism. In Journal of psychiatric research, 123, 119-127. doi:10.1016/j.jpsychires.2020.02.001. https://pubmed.ncbi.nlm.nih.gov/32065947/
4. Villa, Osvaldo, Stuhr, Nicole L, Yen, Chia-An, Arpawong, Thalida Em, Curran, Sean P. 2022. Genetic variation in ALDH4A1 is associated with muscle health over the lifespan and across species. In eLife, 11, . doi:10.7554/eLife.74308. https://pubmed.ncbi.nlm.nih.gov/35470798/
5. Holmes, Roger S. 2023. Sequences, phylogeny and evolution of mitochondrial delta-1-pyrroline-5-carboxylate dehydrogenases (ALDH4A1). Evidence for a second locus (ALDH4A2) in Drosophila. In Chemico-biological interactions, 383, 110679. doi:10.1016/j.cbi.2023.110679. https://pubmed.ncbi.nlm.nih.gov/37597643/
6. Bogner, Alexandra N, Stiers, Kyle M, McKay, Cole M, Becker, Donald F, Tanner, John J. 2021. Structural basis for the stereospecific inhibition of the dual proline/hydroxyproline catabolic enzyme ALDH4A1 by trans-4-hydroxy-L-proline. In Protein science : a publication of the Protein Society, 30, 1714-1722. doi:10.1002/pro.4131. https://pubmed.ncbi.nlm.nih.gov/34048122/
7. Tanner, John J. 2017. Structural Biology of Proline Catabolic Enzymes. In Antioxidants & redox signaling, 30, 650-673. doi:10.1089/ars.2017.7374. https://pubmed.ncbi.nlm.nih.gov/28990412/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen