C57BL/6JCya-Zfp598em1flox/Cya
Common Name:
Zfp598-flox
Product ID:
S-CKO-05755
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Zfp598-flox
Strain ID
CKOCMP-213753-Zfp598-B6J-VA
Gene Name
Product ID
S-CKO-05755
Gene Alias
Ntrap; Znf598
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zfp598em1flox/Cya mice (Catalog S-CKO-05755) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000234956
NCBI RefSeq
NM_183149
Target Region
Exon 2~5
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
Zfp598 is a gene whose function was explored in the context of embryonic stem cell (ESC) self-renewal. Although no common aliases are provided in the given reference, it is essential for the self-renewal of ESCs.
In a study where parallel genome-wide Nuclease technology knockout (KO) screens were carried out in ESCs cultured under serum plus LIF (SL) and 2i plus LIF (2iL) conditions, Zfp598 was found to be indispensable for the self-renewal of ESCs in both culture conditions. The knockout of Zfp598 likely disrupts the normal self-renewal process of ESCs, suggesting its crucial role in maintaining the pluripotent state of these cells [1].
In conclusion, Zfp598 is vital for the self-renewal of mouse embryonic stem cells. The gene knockout mouse models in this research clearly demonstrated its essential function in the self-renewal process of ESCs, contributing to the understanding of the molecular regulation of ESC self-renewal in different pluripotency states.
References：
1. Wang, Jiangjun, Zhang, Chen, Huang, Yi, Zhang, Junlei, Jian, Rui. 2023. Parallel Genome-Wide CRISPR Screens to Identify State-Dependent Self-Renewal Regulators of Mouse Embryonic Stem Cells. In Stem cells and development, 32, 450-464. doi:10.1089/scd.2023.0053. https://pubmed.ncbi.nlm.nih.gov/37166379/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen