C57BL/6JCya-Tbl3em1flox/Cya
Common Name:
Tbl3-flox
Product ID:
S-CKO-05759
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Tbl3-flox
Strain ID
CKOCMP-213773-Tbl3-B6J-VA
Gene Name
Product ID
S-CKO-05759
Gene Alias
9430070M15Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tbl3em1flox/Cya mice (Catalog S-CKO-05759) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000126319
NCBI RefSeq
NM_145396
Target Region
Exon 2~12
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
Tbl3, also known as transducin (beta)-like 3, has diverse functions across different organisms. In plants, it is involved in cell wall biosynthesis, specifically in xylan 3-O-acetylation [1]. In zebrafish, it plays a tissue-specific role in regulating cell cycle rate during development [2].
In Arabidopsis, the simultaneous mutation of Tbl3 and Tbl31 led to a reduction in xylan 3-O-acetylation, and the tbl3 tbl31 esk1 triple mutant showed an even more drastic decrease, indicating functional redundancy in xylan 3-O-acetylation [1]. In zebrafish, the absence of the Tbl3 gene in the cey mutant led to a severe reduction in T cells and hematopoietic stem/progenitor cells (HSPCs), as well as other tissues. The progression of both hematopoietic and retinal development was delayed due to a slowing of the cell cycle [2].
In conclusion, Tbl3 is crucial for cell wall-related processes in plants and for regulating the cell cycle during zebrafish development. The use of mutant models in these organisms has been instrumental in revealing these functions, providing insights into the fundamental biological processes that Tbl3 is involved in.
References:
1. Yuan, Youxi, Teng, Quincy, Zhong, Ruiqin, Ye, Zheng-Hua. 2015. TBL3 and TBL31, Two Arabidopsis DUF231 Domain Proteins, are Required for 3-O-Monoacetylation of Xylan. In Plant & cell physiology, 57, 35-45. doi:10.1093/pcp/pcv172. https://pubmed.ncbi.nlm.nih.gov/26556650/
2. Hutchinson, Sarah A, Tooke-Locke, Erin, Wang, Jindong, Katz, Tammisty, Trede, Nikolaus S. 2012. Tbl3 regulates cell cycle length during zebrafish development. In Developmental biology, 368, 261-72. doi:10.1016/j.ydbio.2012.05.024. https://pubmed.ncbi.nlm.nih.gov/22659140/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen