C57BL/6JCya-Thbdem1flox/Cya
Common Name
Thbd-flox
Product ID
S-CKO-06258
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-21824-Thbd-B6J-VA
Status
When using this mouse strain in a publication, please cite “Thbd-flox Mouse (Catalog S-CKO-06258) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Thbd-flox
Strain ID
CKOCMP-21824-Thbd-B6J-VA
Gene Name
Product ID
S-CKO-06258
Gene Alias
TM, CD141
Background
C57BL/6JCya
Gene Full Name
thrombomodulin
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 2 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000099270
NCBI Transcript ID
NM_009378
Target Region
Exon 1
Size of Effective Region
~1.7 kb
Overview of Gene Research
Thbd, also known as thrombomodulin, is a key component of the anticoagulant protein C-system, acting at the endothelium. It is involved in anti-inflammatory and barrier-stabilizing processes, playing a significant role in maintaining hemostatic balance [1].
In a mouse model where the middle cerebral artery was occluded to induce ischemic brain damage, endothelial expression of Thbd increased in the peri-infarct area while decreasing in the core of the ischemic tissue compared to the contralateral side. Inducible deletion of Thbd specifically in brain endothelial cells worsened stroke outcome 48 hours after occlusion, with decreased vessel diameters and impaired angiogenesis in the peri-infarct area, indicating its role in enhancing vessel diameter and proliferation during ischemic stroke [1].
In another study, in mouse models of NASH, inhibiting the Thbd-PAR1 signaling axis with vorapaxar reduced the burden of senescent hepatocytes and improved NASH and fibrosis, suggesting that this signaling axis is a novel therapeutic target for NASH [2].
In conclusion, Thbd is crucial for maintaining hemostasis, anti-inflammation, and endothelial-related functions. Mouse models, especially those with inducible deletion of Thbd, have revealed its significance in ischemic stroke and NASH. These findings provide valuable insights into the role of Thbd in disease processes and potential therapeutic targets.
References：
1. Wenzel, Jan, Spyropoulos, Dimitrios, Assmann, Julian Christopher, Isermann, Berend, Schwaninger, Markus. 2020. Endogenous THBD (Thrombomodulin) Mediates Angiogenesis in the Ischemic Brain-Brief Report. In Arteriosclerosis, thrombosis, and vascular biology, 40, 2837-2844. doi:10.1161/ATVBAHA.120.315061. https://pubmed.ncbi.nlm.nih.gov/33028093/
2. Maeso-Díaz, Raquel, Du, Kuo, Pan, Christopher, Wang, Xiao-Fan, Diehl, Anna Mae. 2023. Targeting senescent hepatocytes using the thrombomodulin-PAR1 inhibitor vorapaxar ameliorates NAFLD progression. In Hepatology (Baltimore, Md.), 78, 1209-1222. doi:10.1097/HEP.0000000000000401. https://pubmed.ncbi.nlm.nih.gov/37036206/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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