C57BL/6NCya-Tlr4em1flox/Cya
Common Name:
Tlr4-flox
Product ID:
S-CKO-06341
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tlr4-flox
Strain ID
CKOCMP-21898-Tlr4-B6N-VA
Gene Name
Product ID
S-CKO-06341
Gene Alias
Lps; Ly87; Ran/M1; Rasl2-8
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Tlr4em1flox/Cya mice (Catalog S-CKO-06341) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000048096
NCBI RefSeq
NM_021297
Target Region
Exon 2
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
Tlr4, a member of the Toll-like receptor (TLR) family, is a transmembrane receptor that plays a central role in the innate immune response. It recognizes lipopolysaccharide (LPS) or bacterial endotoxins, mediating inflammatory responses. Through adaptor proteins like MyD88 and TRIF, it activates transcription factors NF-κB and AP-1, leading to upregulation of pro-inflammatory cytokines and increased interferon expression [1,2].
In a study with TLR4-knockout (TLR4-/-) mice challenged with LPS, deletion of TLR4 attenuated LPS-induced acute liver injury by inhibiting inflammation and apoptosis via the TLR4/MyD88/NF-κB signaling pathway, demonstrating that TLR4 plays a vital role in septic injury [3].
In conclusion, Tlr4 is crucial for the innate immune response and inflammation regulation. TLR4-KO mouse models have shown its significance in septic acute liver injury, highlighting its potential as a therapeutic target for related diseases.
References：
1. Zhang, Yongsheng, Liang, Xinjie, Bao, Xuefei, Xiao, Wei, Chen, Guoliang. 2022. Toll-like receptor 4 (TLR4) inhibitors: Current research and prospective. In European journal of medicinal chemistry, 235, 114291. doi:10.1016/j.ejmech.2022.114291. https://pubmed.ncbi.nlm.nih.gov/35307617/
2. Kim, Hyo-Jin, Kim, Hyemin, Lee, Jeong-Hyung, Hwangbo, Cheol. 2023. Toll-like receptor 4 (TLR4): new insight immune and aging. In Immunity & ageing : I & A, 20, 67. doi:10.1186/s12979-023-00383-3. https://pubmed.ncbi.nlm.nih.gov/38001481/
3. Chen, Sai-Nan, Tan, Ying, Xiao, Xiao-Chan, Ren, Jun, Dong, Mao-Long. 2021. Deletion of TLR4 attenuates lipopolysaccharide-induced acute liver injury by inhibiting inflammation and apoptosis. In Acta pharmacologica Sinica, 42, 1610-1619. doi:10.1038/s41401-020-00597-x. https://pubmed.ncbi.nlm.nih.gov/33495514/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen