C57BL/6JCya-Otulinlem1flox/Cya
Common Name:
Otulinl-flox
Product ID:
S-CKO-06600
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Otulinl-flox
Strain ID
CKOCMP-223433-Otulinl-B6J-VA
Gene Name
Product ID
S-CKO-06600
Gene Alias
9830126M18; Fam105a
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Otulinlem1flox/Cya mice (Catalog S-CKO-06600) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000226145
NCBI RefSeq
NM_001242423
Target Region
Exon 2~4
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
Otulinl, also known as FAM105A, is the only putative pseudodeubiquitinase of the ovarian tumor protease (OTU domain) family in humans. It lacks deubiquitinase activity but may function through mediating protein-protein interactions. It localizes to the endoplasmic reticulum (ER) and nuclear envelope, and its interactome shows enrichment in proteins from ER/outer nuclear, Golgi and vesicular membranes [1].
The crystal structure of FAM105A (Otulinl) revealed structural deficiencies in the active site and substrate-binding infrastructure, which impairs normal deubiquitinase (DUB) function. It has no catalytic function against all ubiquitin linkages and cannot bind ubiquitin compared with catalytically active FAM105B/OTULIN [1].
In conclusion, Otulinl is a pseudodeubiquitinase that localizes to the ER membrane and likely functions through protein-protein interactions rather than deubiquitinase activity. Although the reference does not mention KO/CKO mouse models, understanding its non-catalytic functions can potentially contribute to insights in areas related to cell fate control and ubiquitin-mediated cellular processes [1,2].
References：
1. Ceccarelli, Derek F, Ivantsiv, Sofiia, Mullin, Amber Anne, Cordes, Sabine, Sicheri, Frank. 2019. FAM105A/OTULINL Is a Pseudodeubiquitinase of the OTU-Class that Localizes to the ER Membrane. In Structure (London, England : 1993), 27, 1000-1012.e6. doi:10.1016/j.str.2019.03.022. https://pubmed.ncbi.nlm.nih.gov/31056421/
2. Weinelt, Nadine, van Wijk, Sjoerd J L. 2020. Ubiquitin-dependent and -independent functions of OTULIN in cell fate control and beyond. In Cell death and differentiation, 28, 493-504. doi:10.1038/s41418-020-00675-x. https://pubmed.ncbi.nlm.nih.gov/33288901/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen