C57BL/6JCya-Selenooem1flox/Cya
Common Name:
Selenoo-flox
Product ID:
S-CKO-06661
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Selenoo-flox
Strain ID
CKOCMP-223776-Selenoo-B6J-VA
Gene Name
Product ID
S-CKO-06661
Gene Alias
1300018J18Rik; Selo
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Selenooem1flox/Cya mice (Catalog S-CKO-06661) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000082439
NCBI RefSeq
NM_027905
Target Region
Exon 2~3
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
Selenoo, also known as selenoprotein O and SELO, is an evolutionarily conserved selenoprotein. It catalyzes a posttranslational protein modification called AMPylation, which is essential for the oxidative stress response [2]. SELENOO localizes to the mitochondria and AMPylates proteins involved in redox homeostasis [2].
In a murine model of spontaneous melanoma metastasis, SELENOO deficiency significantly reduced metastasis to distant visceral organs [1]. This effect could be rescued by treatment with the antioxidant N-acetylcysteine [1]. Mechanistically, SELENOO AMPylated succinate dehydrogenase subunit A, one of the key subunits of mitochondrial complex II, and SELENOO-deficient cells featured increased mitochondrial complex II activity [1].
In conclusion, SELENOO plays a crucial role in melanoma metastasis by modulating mitochondrial function and oxidative stress. The study of SELENOO-deficient murine models has provided valuable insights into its role in cancer progression, highlighting the impact of SELENOO-mediated posttranslational modification of mitochondrial substrates in this disease area [1].
References：
1. Nascentes Melo, Luiza Martins, Sabatier, Marie, Ramesh, Vijayashree, Sreelatha, Anju, Tasdogan, Alpaslan. . Selenoprotein O Promotes Melanoma Metastasis and Regulates Mitochondrial Complex II Activity. In Cancer research, 85, 942-955. doi:10.1158/0008-5472.CAN-23-2194. https://pubmed.ncbi.nlm.nih.gov/39700395/
2. Sreelatha, Anju, Yee, Samantha S, Lopez, Victor A, Tomchick, Diana R, Tagliabracci, Vincent S. 2018. Protein AMPylation by an Evolutionarily Conserved Pseudokinase. In Cell, 175, 809-821.e19. doi:10.1016/j.cell.2018.08.046. https://pubmed.ncbi.nlm.nih.gov/30270044/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen