C57BL/6JCya-Selenooem1flox/Cya
Common Name
Selenoo-flox
Product ID
S-CKO-06661
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-223776-Selenoo-B6J-VA
When using this mouse strain in a publication, please cite “Selenoo-flox Mouse (Catalog S-CKO-06661) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Selenoo-flox
Strain ID
CKOCMP-223776-Selenoo-B6J-VA
Gene Name
Product ID
S-CKO-06661
Gene Alias
1300018J18Rik, Selo
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 15
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000082439
NCBI RefSeq
NM_027905
Target Region
Exon 2~3
Size of Effective Region
~2.3 kb
Overview of Gene Research
Selenoo, also known as selenoprotein O and SELO, is an evolutionarily conserved selenoprotein. It catalyzes a posttranslational protein modification called AMPylation, which is essential for the oxidative stress response [2]. SELENOO localizes to the mitochondria and AMPylates proteins involved in redox homeostasis [2].
In a murine model of spontaneous melanoma metastasis, SELENOO deficiency significantly reduced metastasis to distant visceral organs [1]. This effect could be rescued by treatment with the antioxidant N-acetylcysteine [1]. Mechanistically, SELENOO AMPylated succinate dehydrogenase subunit A, one of the key subunits of mitochondrial complex II, and SELENOO-deficient cells featured increased mitochondrial complex II activity [1].
In conclusion, SELENOO plays a crucial role in melanoma metastasis by modulating mitochondrial function and oxidative stress. The study of SELENOO-deficient murine models has provided valuable insights into its role in cancer progression, highlighting the impact of SELENOO-mediated posttranslational modification of mitochondrial substrates in this disease area [1].
References：
1. Nascentes Melo, Luiza Martins, Sabatier, Marie, Ramesh, Vijayashree, Sreelatha, Anju, Tasdogan, Alpaslan. . Selenoprotein O Promotes Melanoma Metastasis and Regulates Mitochondrial Complex II Activity. In Cancer research, 85, 942-955. doi:10.1158/0008-5472.CAN-23-2194. https://pubmed.ncbi.nlm.nih.gov/39700395/
2. Sreelatha, Anju, Yee, Samantha S, Lopez, Victor A, Tomchick, Diana R, Tagliabracci, Vincent S. 2018. Protein AMPylation by an Evolutionarily Conserved Pseudokinase. In Cell, 175, 809-821.e19. doi:10.1016/j.cell.2018.08.046. https://pubmed.ncbi.nlm.nih.gov/30270044/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.