C57BL/6JCya-Zfp35em1flox/Cya
Common Name
Zfp35-flox
Product ID
S-CKO-07008
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-22694-Zfp35-B6J-VA
When using this mouse strain in a publication, please cite “Zfp35-flox Mouse (Catalog S-CKO-07008) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Zfp35-flox
Strain ID
CKOCMP-22694-Zfp35-B6J-VA
Gene Name
Product ID
S-CKO-07008
Gene Alias
Zfp-35, Znf271, mszf13, mszf28, mszf68, mszf20-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 18
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000074941
NCBI RefSeq
NM_011755
Target Region
Exon 3
Size of Effective Region
~2.4 kb
Overview of Gene Research
Zfp35, a Kruppel-type zinc finger protein-encoding gene, contains 18 zinc finger motifs. Zinc finger proteins are regulatory transcription factors crucial for cellular development and function [1,2,3].
In Zfp35-deficient (Zfp35(-/-)) mice, Th2 cell differentiation, Th2 cytokine production, eosinophilic inflammation, and airway hyperresponsiveness were enhanced. Adoptive transfer of Ag-sensitized Zfp35(-/-) CD4 T cells into asthmatic mice also led to increased airway inflammation and hyperresponsiveness. These results suggest Zfp35 negatively controls Th2 cell differentiation, allergic airway inflammation, and airway hyperresponsiveness [1]. Additionally, Zfp35 is transiently expressed during spermatogenesis, specifically up-regulated at the pachytene stage of meiotic prophase in spermatocytes [2,3].
In conclusion, Zfp35 plays important roles in controlling Th2-dependent allergic inflammation, such as in allergic asthma, and in regulating gene activity during spermatogenesis. The gene knockout mouse models have been instrumental in revealing these functions, providing insights into the mechanisms underlying allergic diseases and spermatogenesis [1,2,3].
References：
1. Kitajima, Masayuki, Iwamura, Chiaki, Miki-Hosokawa, Takako, Yamashita, Masakatsu, Nakayama, Toshinori. 2009. Enhanced Th2 cell differentiation and allergen-induced airway inflammation in Zfp35-deficient mice. In Journal of immunology (Baltimore, Md. : 1950), 183, 5388-96. doi:10.4049/jimmunol.0804155. https://pubmed.ncbi.nlm.nih.gov/19783676/
2. Cunliffe, V, Koopman, P, McLaren, A, Trowsdale, J. . A mouse zinc finger gene which is transiently expressed during spermatogenesis. In The EMBO journal, 9, 197-205. doi:. https://pubmed.ncbi.nlm.nih.gov/2104800/
3. Cunliffe, V, Williams, S, Trowsdale, J. . Genomic analysis of a mouse zinc finger gene, Zfp-35, that is up-regulated during spermatogenesis. In Genomics, 8, 331-9. doi:. https://pubmed.ncbi.nlm.nih.gov/2249852/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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