C57BL/6JCya-Smim26em1flox/Cya
Common Name:
Smim26-flox
Product ID:
S-CKO-07184
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Smim26-flox
Strain ID
CKOCMP-228715-Smim26-B6J-VA
Gene Name
Product ID
S-CKO-07184
Gene Alias
Gm561
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Smim26em1flox/Cya mice (Catalog S-CKO-07184) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000136628
NCBI RefSeq
NM_001033297
Target Region
Exon 2
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Smim26 is a microprotein encoded by the lncRNA LINC00493. It localizes in mitochondria and exerts anti-metastatic activity in clear cell renal cell carcinoma (ccRCC) [1]. By interacting with acylglycerol kinase (AGK) and glutathione transport regulator SLC25A11 via its N-terminus, it suppresses ccRCC growth and metastatic lung colonization, inhibits AGK-mediated AKT phosphorylation, and maintains mitochondrial glutathione import and respiratory efficiency [1].
The knockdown and overexpression experiments suggest that both LINC00493/SMIM26 transcript and protein affect cell viability, but in an opposite manner [2].
In conclusion, Smim26 is a significant microprotein encoded by lncRNA LINC00493, which plays a crucial anti-metastatic role in ccRCC and has an impact on cell viability. Its study helps in understanding the underlying mechanisms of cancer metastasis and cell physiological regulation.
References：
1. Meng, Kun, Lu, Shaohua, Li, Yu-Ying, Zhang, Chris Zhiyi, He, Qing-Yu. 2023. LINC00493-encoded microprotein SMIM26 exerts anti-metastatic activity in renal cell carcinoma. In EMBO reports, 24, e56282. doi:10.15252/embr.202256282. https://pubmed.ncbi.nlm.nih.gov/37009826/
2. Konina, Daria, Sparber, Peter, Viakhireva, Iuliia, Filatova, Alexandra, Skoblov, Mikhail. 2021. Investigation of LINC00493/SMIM26 Gene Suggests Its Dual Functioning at mRNA and Protein Level. In International journal of molecular sciences, 22, . doi:10.3390/ijms22168477. https://pubmed.ncbi.nlm.nih.gov/34445188/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen