C57BL/6JCya-Bmal2em1flox/Cya
Common Name:
Bmal2-flox
Product ID:
S-CKO-09971
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Bmal2-flox
Strain ID
CKOCMP-272322-Bmal2-B6J-VA
Gene Name
Product ID
S-CKO-09971
Gene Alias
4632430A05Rik; Arntl2; CLIF; MOP9; bHLHe6
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Bmal2em1flox/Cya mice (Catalog S-CKO-09971) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000080530
NCBI RefSeq
NM_172309
Target Region
Exon 4
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Bmal2, also known as ARNTL2, is a member of the basic helix-loop-helix PER-ARNT-SIM family of transcription factors. It forms heterodimers with CLOCK and NPAS2 to activate the transcription of clock and clock-controlled genes, playing a crucial role in regulating circadian rhythms. It is also involved in various biological processes such as metabolism, immune response, and cell proliferation, making it an important gene for understanding physiological and pathological conditions. Gene knockout models, like the Bmal2 knockout (B2KO) mouse model, are valuable tools for studying its functions [2,3].
In B2KO male mice, there are significant alterations. They show increased adiposity and fasted hyperinsulinemia, with lower energy expenditure at night. The master clock in the suprachiasmatic nuclei is impaired, as indicated by a shorter free-running period and loss of daily rhythmicity in the expression of intracellular metabolic regulators. The circadian window of eating is longer, and the circadian patterns of food intake and meal numbers change. Also, food-anticipatory activity is almost prevented, suggesting an altered food clock. In the mediobasal hypothalamus of B2KO mice, the expression of orexigenic neuropeptide-coding genes is downregulated, and Lipoprotein lipase expression loses its rhythmicity [2]. In pancreatic cancer cell lines, knockout of Bmal2 affects cell viability, invasion, glycolysis, and other metabolic processes under hypoxic conditions. Bmal2 directly regulates hypoxia-responsive target genes and is necessary for the stabilization of HIF1A and destabilization of HIF2A upon exposure to hypoxia [1].
In conclusion, Bmal2 is essential for maintaining normal circadian regulation of metabolism, feeding behavior, and energy balance as revealed by the B2KO mouse model. It also plays a significant role in hypoxic metabolism in pancreatic cancer. Understanding Bmal2 through such model-based research provides insights into the mechanisms of metabolic disorders and cancer, potentially guiding the development of new therapeutic strategies.
References:
1. Maurer, H Carlo, Garcia-Curiel, Alvaro, Holmstrom, Sam R, Califano, Andrea, Olive, Kenneth P. 2024. Ras-dependent activation of BMAL2 regulates hypoxic metabolism in pancreatic cancer. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.03.19.533333. https://pubmed.ncbi.nlm.nih.gov/36993718/
2. Dantas-Ferreira, Rosana, Ciocca, Dominique, Vuillez, Patrick, Rogner, Ute C, Challet, Etienne. 2024. Deletion of the Clock Gene Bmal2 Leads to Alterations in Hypothalamic Clocks, Circadian Regulation of Feeding, and Energy Balance. In The Journal of neuroscience : the official journal of the Society for Neuroscience, 44, . doi:10.1523/JNEUROSCI.1886-23.2024. https://pubmed.ncbi.nlm.nih.gov/38531632/
3. Schoenhard, John A, Eren, Mesut, Johnson, Carl H, Vaughan, Douglas E. . Alternative splicing yields novel BMAL2 variants: tissue distribution and functional characterization. In American journal of physiology. Cell physiology, 283, C103-14. doi:. https://pubmed.ncbi.nlm.nih.gov/12055078/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen