C57BL/6JCya-Trpm2em1flox/Cya
Common Name:
Trpm2-flox
Product ID:
S-CKO-10151
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Trpm2-flox
Strain ID
CKOCMP-28240-Trpm2-B6J-VA
Gene Name
Product ID
S-CKO-10151
Gene Alias
9830168K16Rik; LTRPC2; TRPC7; Trp7; Trrp7
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Trpm2em1flox/Cya mice (Catalog S-CKO-10151) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000105401
NCBI RefSeq
NM_138301
Target Region
Exon 4
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Trpm2, short for transient receptor potential melastatin 2, is an oxidant-activated non-selective cation channel. It is widely expressed in mammalian tissues. Oxidative stress stimulates intracellular ADP-ribose formation which opens TRPM2 channels, acting as an endogenous redox sensor for mediating oxidative stress/ROS-induced Ca(2+) entry and subsequent Ca(2+)-dependent cellular reactions like endothelial hyperpermeability and apoptosis [2].
In a study on acute myocardial infarction (AMI) in mice, knockout of TRPM2 protected against myocardial injury. Myocardial infarction was induced by ligation of the left anterior descending coronary artery in wild-type and TRPM2 knockout (TRPM2-/-) male mice. Knockout of TRPM2 led to up-regulation of clusterin, suggesting an interaction between the two in AMI [1].
In conclusion, Trpm2 is crucial in mediating oxidative stress-related Ca(2+) entry and cellular responses. The use of TRPM2 knockout mouse models has revealed its role in myocardial injury, specifically in the context of its interaction with clusterin in AMI, providing insights into potential treatment strategies for such cardiac conditions.
References：
1. Li, Dalei, Wang, Mengying, Fan, Rong, Gan, Hailin, Fan, Huaying. 2023. Clusterin regulates TRPM2 to protect against myocardial injury induced by acute myocardial infarction injury. In Tissue & cell, 82, 102038. doi:10.1016/j.tice.2023.102038. https://pubmed.ncbi.nlm.nih.gov/36870313/
2. Hecquet, Claudie M, Malik, Asrar B. . Role of H(2)O(2)-activated TRPM2 calcium channel in oxidant-induced endothelial injury. In Thrombosis and haemostasis, 101, 619-25. doi:. https://pubmed.ncbi.nlm.nih.gov/19350103/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen