C57BL/6JCya-Nit2em1flox/Cya
Common Name:
Nit2-flox
Product ID:
S-CKO-11536
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Nit2-flox
Strain ID
CKOCMP-52633-Nit2-B6J-VA
Gene Name
Product ID
S-CKO-11536
Gene Alias
1190017B19Rik; D16Ertd502e
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nit2em1flox/Cya mice (Catalog S-CKO-11536) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000023432
NCBI RefSeq
NM_023175
Target Region
Exon 5~6
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Nit2, also known as nitrilase family member 2, has diverse functions. In humans, it's involved in chemoresistance in gastric cancer [1]. In organisms like Neurospora crassa and Chlamydomonas reinhardtii, it serves as a key transcription factor regulating genes related to nitrogen metabolism [3,5].
In gastric cancer, depletion or low expression of Nit2 led to 5-Fluorouracil (5-FU) resistance. Mechanistically, Nit2 interacts with bromodomain-containing protein 1 (BRD1) to inhibit histone acetylation and oxidative phosphorylation gene expression [1]. In tongue squamous cell carcinoma, its overexpression predicts poor prognosis [2]. In colon cancer cell lines, downregulation of Nit2 inhibits cell proliferation and induces cell cycle arrest through caspase-3 and PARP pathways [4].
In conclusion, Nit2 plays crucial roles in multiple biological processes and disease conditions. Its function in chemoresistance in gastric cancer, and its prognostic value in tongue squamous cell carcinoma, along with its impact on colon cancer cell behavior, highlight its significance in cancer research. Understanding Nit2 can potentially offer new therapeutic strategies for these cancers.
References：
1. Wang, Ziyang, Di, Yuqin, Wen, Xiangqiong, Wang, Xiongjun, He, Weiling. 2024. NIT2 dampens BRD1 phase separation and restrains oxidative phosphorylation to enhance chemosensitivity in gastric cancer. In Science translational medicine, 16, eado8333. doi:10.1126/scitranslmed.ado8333. https://pubmed.ncbi.nlm.nih.gov/39565874/
2. Chen, Shan, Wang, Zengyan, Feng, Chongjin. 2020. NIT2 overexpression predicts poor prognosis in tongue squamous cell carcinoma patients. In Molecular biology reports, 47, 1553-1561. doi:10.1007/s11033-019-05197-5. https://pubmed.ncbi.nlm.nih.gov/31925645/
3. Tao, Y, Marzluf, G A. . The NIT2 nitrogen regulatory protein of Neurospora: expression and stability of nit-2 mRNA and protein. In Current genetics, 36, 153-8. doi:. https://pubmed.ncbi.nlm.nih.gov/10501938/
4. Zheng, Bo'an, Chai, Rui, Yu, Xiaojun. 2015. Downregulation of NIT2 inhibits colon cancer cell proliferation and induces cell cycle arrest through the caspase-3 and PARP pathways. In International journal of molecular medicine, 35, 1317-22. doi:10.3892/ijmm.2015.2125. https://pubmed.ncbi.nlm.nih.gov/25738796/
5. Camargo, Antonio, Llamas, Angel, Schnell, Rogene A, Fernández, Emilio, Galván, Aurora. 2007. Nitrate signaling by the regulatory gene NIT2 in Chlamydomonas. In The Plant cell, 19, 3491-503. doi:. https://pubmed.ncbi.nlm.nih.gov/18024571/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen