C57BL/6NCya-Isg20em1flox/Cya
Common Name:
Isg20-flox
Product ID:
S-CKO-12340
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Isg20-flox
Strain ID
CKOCMP-57444-Isg20-B6N-VA
Gene Name
Product ID
S-CKO-12340
Gene Alias
1600023I01Rik; 2010107M23Rik; 20kDa; DnaQL; HEM45
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Isg20em1flox/Cya mice (Catalog S-CKO-12340) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000121645
NCBI RefSeq
NM_001291220
Target Region
Exon 3~4
Size of Effective Region
~4.7 kb
Detailed Document
Overview of Gene Research
Isg20, also known as HEM45 (HeLa estrogen-modulated, band 45), is an interferon-stimulated gene encoding a 20-kDa protein. It belongs to the DEDD exonucleases family and has RNase properties, playing a role in mediating interferon's antiviral activities. It can cleave single-stranded RNA and DNA, and its expression can be induced by type I and II interferons [3].
In terms of functional studies, Isg20 has been shown to inhibit the replication of various viruses. For example, overexpression of ovine Isg20 (oIsg20) suppressed bluetongue virus (BTV) replication, while knockdown increased it. oIsg20 interacted with BTV protein VP4, and exonuclease-defective oIsg20 had a decreased inhibitory effect on BTV replication [1]. In acute kidney injury (AKI), tubule-specific deficiency of Isg20 in mice aggravated renal injury and RNA oxidation, while overexpression protected against oxidized RNA overproduction and renal ischemia/reperfusion injury, indicating Isg20's role in regulating RNA oxidation in AKI [2].
In conclusion, Isg20 is an important gene involved in antiviral defense and the regulation of RNA oxidation in AKI. Studies using gene knockout or conditional knockout models in mice have provided insights into its role in these biological processes and disease conditions, which may help in developing new therapeutic strategies for viral infections and AKI.
References：
1. Kang, Di, Gao, Shandian, Tian, Zhancheng, Du, Junzheng, Yin, Hong. 2022. ISG20 inhibits bluetongue virus replication. In Virologica Sinica, 37, 521-530. doi:10.1016/j.virs.2022.04.010. https://pubmed.ncbi.nlm.nih.gov/35513266/
2. Jia, Meng, Li, Liang, Chen, Ruiqi, Tang, Wei, Yi, Fan. 2023. Targeting RNA oxidation by ISG20-mediated degradation is a potential therapeutic strategy for acute kidney injury. In Molecular therapy : the journal of the American Society of Gene Therapy, 31, 3034-3051. doi:10.1016/j.ymthe.2023.07.008. https://pubmed.ncbi.nlm.nih.gov/37452495/
3. Zheng, Zhiwei, Wang, Lin, Pan, Jihong. . Interferon-stimulated gene 20-kDa protein (ISG20) in infection and disease: Review and outlook. In Intractable & rare diseases research, 6, 35-40. doi:10.5582/irdr.2017.01004. https://pubmed.ncbi.nlm.nih.gov/28357179/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen