C57BL/6JCya-Chst11em1flox/Cya
Common Name
Chst11-flox
Product ID
S-CKO-12431
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-58250-Chst11-B6J-VA
Status
When using this mouse strain in a publication, please cite “Chst11-flox Mouse (Catalog S-CKO-12431) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Chst11-flox
Strain ID
CKOCMP-58250-Chst11-B6J-VA
Gene Name
Product ID
S-CKO-12431
Gene Alias
C4s, C4ST, C4ST1, C4ST-1, 1110020P09Rik
Background
C57BL/6JCya
Gene Full Name
carbohydrate sulfotransferase 11
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 10 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000040110
NCBI Transcript ID
NM_021439
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Overview of Gene Research
Chst11, or carbohydrate (chondroitin 4) sulfotransferase-11, is a Golgi membrane protein that catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine residues of chondroitin. Chondroitin sulfate, whose sulfation is mediated by Chst11, is a predominant proteoglycan in cartilage, playing a crucial role in the developing growth plate of cartilage [5]. It is also involved in various biological processes and diseases through its impact on chondroitin sulfate modification.
Chst11-deficient mouse models have severe chondrodysplasia, congenital arthritis and neonatal lethality, highlighting its crucial role in skeletal morphogenesis [5]. In human studies, a homozygous deletion in CHST11 was found in a consanguineous Pakistani kindred with limb malformations and skeletal defects, further confirming its role in skeletal development [5]. In cancer research, CHST11 is frequently overexpressed in glioblastoma, promoting cell mobility. Targeting CHST11-derived chondroitin 4-sulfate with a specific binding peptide improved the survival rate of orthotopic glioma cell transplant mice [1]. In lung cancer and pulmonary fibrosis, CHST11 levels are elevated, and it contributes to tumor malignancy and fibrotic activator promotion. Analyses suggest the CHST11/integrin axis could be a therapeutic target [2]. In pancreatic cancer, high CHST11 expression correlates with poor prognosis and tumor immune infiltration [3]. In breast cancer, its expression is higher in basal-like and Her2-amplified cell lines, and is controlled by DNA methylation [4]. In clear cell renal cell carcinoma, CHST11 promotes cell proliferation, migration and invasion [6].
In conclusion, Chst11 is essential for skeletal development as demonstrated by animal models. In disease areas, especially cancer, its abnormal expression is associated with tumor progression, immune infiltration and prognosis, making it a potential biomarker and therapeutic target. The study of Chst11 knockout mouse models has provided valuable insights into its function in normal development and disease conditions.
References：
1. Lin, You-Cheng, Chu, Yin-Hung, Liao, Wen-Chieh, Yang, Meng-Yin, Liu, Chiung-Hui. 2023. CHST11-modified chondroitin 4-sulfate as a potential therapeutic target for glioblastoma. In American journal of cancer research, 13, 2998-3012. doi:. https://pubmed.ncbi.nlm.nih.gov/37559985/
2. Li, Chien-Hsiu, Chan, Ming-Hsien, Chang, Yu-Chan, Hsiao, Michael. 2022. The CHST11 gene is linked to lung cancer and pulmonary fibrosis. In The journal of gene medicine, 24, e3451. doi:10.1002/jgm.3451. https://pubmed.ncbi.nlm.nih.gov/36181245/
3. Zhang, Pan, Chen, Dan, Cui, Haimeng, Luo, Qingfeng. . High Expression of CHST11 Correlates with Poor Prognosis and Tumor Immune Infiltration of Pancreatic Cancer. In Clinical laboratory, 68, . doi:10.7754/Clin.Lab.2022.211239. https://pubmed.ncbi.nlm.nih.gov/36546737/
4. Herman, Damir, Leakey, Tatiana I, Behrens, Alice, Kieber-Emmons, Thomas, Monzavi-Karbassi, Behjatolah. 2015. CHST11 gene expression and DNA methylation in breast cancer. In International journal of oncology, 46, 1243-51. doi:10.3892/ijo.2015.2828. https://pubmed.ncbi.nlm.nih.gov/25586191/
5. Shabbir, Rana Muhammad Kamran, Nalbant, Gökhan, Ahmad, Nafees, Malik, Sajid, Tolun, Aslıhan. 2018. Homozygous CHST11 mutation in chondrodysplasia, brachydactyly, overriding digits, clino-symphalangism and synpolydactyly. In Journal of medical genetics, 55, 489-496. doi:10.1136/jmedgenet-2017-105003. https://pubmed.ncbi.nlm.nih.gov/29514872/
6. Hu, Weijing, Chen, Yongquan, Zhang, Lin, Wang, Dongwen, Wu, Bo. 2024. Effect of CHST11, a novel biomarker, on the biological functionalities of clear cell renal cell carcinoma. In Scientific reports, 14, 7704. doi:10.1038/s41598-024-58280-8. https://pubmed.ncbi.nlm.nih.gov/38565604/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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