C57BL/6JCya-Ghrlem1flox/Cya
Common Name:
Ghrl-flox
Product ID:
S-CKO-12460
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ghrl-flox
Strain ID
CKOCMP-58991-Ghrl-B6J-VA
Gene Name
Product ID
S-CKO-12460
Gene Alias
2210006E23Rik; Ghr; MTLRP; MTLRPAP; m46
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ghrlem1flox/Cya mice (Catalog S-CKO-12460) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000064993
NCBI RefSeq
NM_021488
Target Region
Exon 4
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
GHRL, also known as the ghrelin prepropeptide gene, encodes the protein ghrelin which regulates appetite and energy balance in the human body [2]. Ghrelin is involved in multiple physiological processes and is associated with pathways related to metabolism, immune regulation, and potentially carcinogenesis [2]. Genetic models are valuable for studying GHRL's role in health and disease.
A meta-analysis involving 8,194 participants found that the GHRL gene Leu72Met polymorphism was significantly correlated with an increased risk of type 2 diabetes mellitus (T2DM), especially in the Chinese population [1]. In the Ukrainian population, the T allele of the GHRL (rs696217) polymorphism indicated an increased risk of obesity development [3]. Also, GHRL expression was lower in gastric cancer samples compared to normal samples, and it was correlated with progression of precancerous lesions in gastric cancer [2]. However, a case-control study showed no significant association of GHRL polymorphisms with prostate cancer [4].
In conclusion, GHRL plays a crucial role in metabolism-related processes and disease development, such as in T2DM, obesity, and gastric cancer. The study of GHRL gene polymorphisms in different populations and its expression in various diseases through genetic models helps in understanding the molecular mechanisms underlying these conditions, providing insights for potential diagnostic and therapeutic strategies.
References：
1. Li, Yan-Yan, Lu, Xin-Zheng, Yang, Xin-Xing, Kim, Hyun Jun, Yang, Zhi-Jian. 2019. GHRL Gene Leu72Met Polymorphism and Type 2 Diabetes Mellitus: A Meta-Analysis Involving 8,194 Participants. In Frontiers in endocrinology, 10, 559. doi:10.3389/fendo.2019.00559. https://pubmed.ncbi.nlm.nih.gov/31440212/
2. Wang, Jinyun, Liu, Dingwei, Xie, Yong. 2023. GHRL as a prognostic biomarker correlated with immune infiltrates and progression of precancerous lesions in gastric cancer. In Frontiers in oncology, 13, 1142017. doi:10.3389/fonc.2023.1142017. https://pubmed.ncbi.nlm.nih.gov/37469414/
3. Prodan, Andrii, Dzubanovsky, Ihor, Kamyshnyi, Oleksandr, Grytsenko, Stepan, Voloshyn, Stanislava. 2023. Effect of the GHRL gene (rs696217) polymorphism on the metabolic disorders in patients with obesity in the Ukrainian population. In Endocrine regulations, 57, 173-182. doi:10.2478/enr-2023-0021. https://pubmed.ncbi.nlm.nih.gov/37715984/
4. Merabet, Nesrine, Ramoz, Nicolas, Boulmaiz, Amel, Tolle, Virginie, Berredjem, Hajira. 2023. SNPs-Panel Polymorphism Variations in GHRL and GHSR Genes Are Not Associated with Prostate Cancer. In Biomedicines, 11, . doi:10.3390/biomedicines11123276. https://pubmed.ncbi.nlm.nih.gov/38137497/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen