C57BL/6JCya-Nek7em1flox/Cya
Common Name:
Nek7-flox
Product ID:
S-CKO-12519
Background:
C57BL/6JCya
Product Type
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Genotype
Sex
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Basic Information
Strain Name
Nek7-flox
Strain ID
CKOCMP-59125-Nek7-B6J-VA
Gene Name
Product ID
S-CKO-12519
Gene Alias
2810460C19Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nek7em1flox/Cya mice (Catalog S-CKO-12519) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000186017
NCBI RefSeq
NM_021605
Target Region
Exon 6~7
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
NEK7, short for NIMA-related kinase 7, is a serine/threonine kinase and the smallest one in the mammalian NEK family. It plays a crucial role in regulating the cell cycle, especially in promoting the mitotic process of cells. More importantly, NEK7 is significantly involved in the activation of the NLRP3 inflammasome, which is a key mediator of the innate immune response. Genetic models, such as gene knockout mouse models, have been essential in studying NEK7's functions [1].
In gene knockout studies, mouse chimaeras reconstituted with Nek7(-/-) haematopoietic cells demonstrated that NEK7 is required for NLRP3 inflammasome activation in vivo. Without NEK7, caspase-1 activation and IL-1β release in response to NLRP3-activating signals were abolished [3]. In addition, NEK7 knockdown in vitro abolished ATP + LPS-induced pyroptosis and improved DSS-induced chronic colitis in vivo, suggesting its role in modulating pyroptosis in inflammatory bowel disease [2].
In conclusion, NEK7 has dual functions in regulating the cell cycle and activating the NLRP3 inflammasome. Studies using NEK7 KO mouse models have revealed its significance in inflammatory-related diseases like inflammatory bowel disease, highlighting its potential as a therapeutic target for NLRP3-related diseases [1,2,3].
References：
1. Wang, Jin, Chen, Simeng, Liu, Min, Zhang, Min, Jia, Xiaoyi. 2022. NEK7: a new target for the treatment of multiple tumors and chronic inflammatory diseases. In Inflammopharmacology, 30, 1179-1187. doi:10.1007/s10787-022-01026-7. https://pubmed.ncbi.nlm.nih.gov/35829941/
2. Chen, Xueliang, Liu, Ganglei, Yuan, Yuanyuan, Wang, Shalong, Yuan, Lianwen. 2019. NEK7 interacts with NLRP3 to modulate the pyroptosis in inflammatory bowel disease via NF-κB signaling. In Cell death & disease, 10, 906. doi:10.1038/s41419-019-2157-1. https://pubmed.ncbi.nlm.nih.gov/31787755/
3. He, Yuan, Zeng, Melody Y, Yang, Dahai, Motro, Benny, Núñez, Gabriel. 2016. NEK7 is an essential mediator of NLRP3 activation downstream of potassium efflux. In Nature, 530, 354-7. doi:10.1038/nature16959. https://pubmed.ncbi.nlm.nih.gov/26814970/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen