Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Nek6em1flox/Cya
Common Name:
Nek6-flox
Product ID:
S-CKO-12520
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Nek6-flox
Strain ID
CKOCMP-59126-Nek6-B6J-VA
Gene Name
Nek6
Product ID
S-CKO-12520
Gene Alias
1300007C09Rik
Background
C57BL/6JCya
NCBI ID
59126
Modification
Conditional knockout
Chromosome
2
Phenotype
MGI:1891638
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nek6em1flox/Cya mice (Catalog S-CKO-12520) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000054234
NCBI RefSeq
NM_021606.3
Target Region
Exon 6~7
Size of Effective Region
~1.4 kb
Detailed Document
Click here to download >>
Overview of Gene Research
NEK6, a NIMA-related serine/threonine protein kinase, is essential for mitosis progression, including mitotic spindle formation, metaphase-to-anaphase transition, and centrosome separation. It also has a role in DNA repair and can induce apoptosis when inhibited. It is involved in multiple pathways such as those related to cell cycle, and is of great biological importance in various cellular processes [1].

Knock-out of NEK6 in DU-145 prostate cancer cells decreases clonogenic capacity, proliferation, cell viability, and mitochondrial activity. It increases intracellular ROS levels, decreases antioxidant defenses, increases JNK phosphorylation and DNA damage markers, and induces cell death by apoptosis, making cells more sensitive to cisplatin. Also, in ovarian cancer, inhibiting NEK6 leads to decreased de novo purine synthesis and diminished chemoresistance. In osteosarcoma, down-regulation of NEK6 by siRNAs or miR-26a-5p inhibits cell proliferation, migration, and invasion while promoting apoptosis [3,2,4].

In conclusion, NEK6 is crucial for mitosis and DNA repair. Model-based research, especially gene knockout in cancer cell lines, reveals its significant roles in cancer-related processes such as chemoresistance, redox balance, and cell survival in prostate, ovarian, and osteosarcoma cancers. Understanding NEK6 may provide new strategies for cancer therapy.

References:
1. Panchal, Nagesh Kishan, Mohanty, Shruti, Prince, Sabina Evan. 2022. NIMA-related kinase-6 (NEK6) as an executable target in cancer. In Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico, 25, 66-77. doi:10.1007/s12094-022-02926-4. https://pubmed.ncbi.nlm.nih.gov/36074296/
2. Liu, Jingchun, Wang, Haoyu, Wan, Huanzhi, Yang, Lian, Hong, Li. 2024. NEK6 dampens FOXO3 nuclear translocation to stabilize C-MYC and promotes subsequent de novo purine synthesis to support ovarian cancer chemoresistance. In Cell death & disease, 15, 661. doi:10.1038/s41419-024-07045-2. https://pubmed.ncbi.nlm.nih.gov/39256367/
3. Pavan, Isadora Carolina Betim, Basei, Fernanda Luisa, Severino, Matheus Brandemarte, Simabuco, Fernando Moreira, Kobarg, Jörg. 2023. NEK6 Regulates Redox Balance and DNA Damage Response in DU-145 Prostate Cancer Cells. In Cells, 12, . doi:10.3390/cells12020256. https://pubmed.ncbi.nlm.nih.gov/36672191/
4. Zhu, Min, Sun, Yuyu, Xue, Huawei, Gu, Baorong, Yan, Xiaoling. 2023. NEK6 Promotes the Progression of Osteosarcoma Through Activating STAT3 Signaling Pathway by Down-Regulation of miR-26a-5p. In International journal of general medicine, 16, 2831-2848. doi:10.2147/IJGM.S413461. https://pubmed.ncbi.nlm.nih.gov/37426517/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest