C57BL/6JCya-Nek6em1flox/Cya
Common Name:
Nek6-flox
Product ID:
S-CKO-12520
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Nek6-flox
Strain ID
CKOCMP-59126-Nek6-B6J-VA
Gene Name
Product ID
S-CKO-12520
Gene Alias
1300007C09Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nek6em1flox/Cya mice (Catalog S-CKO-12520) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000054234
NCBI RefSeq
NM_021606.3
Target Region
Exon 6~7
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
NEK6, a NIMA-related serine/threonine protein kinase, is essential for mitosis progression, including mitotic spindle formation, metaphase-to-anaphase transition, and centrosome separation. It also has a role in DNA repair and can induce apoptosis when inhibited. It is involved in multiple pathways such as those related to cell cycle, and is of great biological importance in various cellular processes [1].
Knock-out of NEK6 in DU-145 prostate cancer cells decreases clonogenic capacity, proliferation, cell viability, and mitochondrial activity. It increases intracellular ROS levels, decreases antioxidant defenses, increases JNK phosphorylation and DNA damage markers, and induces cell death by apoptosis, making cells more sensitive to cisplatin. Also, in ovarian cancer, inhibiting NEK6 leads to decreased de novo purine synthesis and diminished chemoresistance. In osteosarcoma, down-regulation of NEK6 by siRNAs or miR-26a-5p inhibits cell proliferation, migration, and invasion while promoting apoptosis [3,2,4].
In conclusion, NEK6 is crucial for mitosis and DNA repair. Model-based research, especially gene knockout in cancer cell lines, reveals its significant roles in cancer-related processes such as chemoresistance, redox balance, and cell survival in prostate, ovarian, and osteosarcoma cancers. Understanding NEK6 may provide new strategies for cancer therapy.
References:
1. Panchal, Nagesh Kishan, Mohanty, Shruti, Prince, Sabina Evan. 2022. NIMA-related kinase-6 (NEK6) as an executable target in cancer. In Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico, 25, 66-77. doi:10.1007/s12094-022-02926-4. https://pubmed.ncbi.nlm.nih.gov/36074296/
2. Liu, Jingchun, Wang, Haoyu, Wan, Huanzhi, Yang, Lian, Hong, Li. 2024. NEK6 dampens FOXO3 nuclear translocation to stabilize C-MYC and promotes subsequent de novo purine synthesis to support ovarian cancer chemoresistance. In Cell death & disease, 15, 661. doi:10.1038/s41419-024-07045-2. https://pubmed.ncbi.nlm.nih.gov/39256367/
3. Pavan, Isadora Carolina Betim, Basei, Fernanda Luisa, Severino, Matheus Brandemarte, Simabuco, Fernando Moreira, Kobarg, Jörg. 2023. NEK6 Regulates Redox Balance and DNA Damage Response in DU-145 Prostate Cancer Cells. In Cells, 12, . doi:10.3390/cells12020256. https://pubmed.ncbi.nlm.nih.gov/36672191/
4. Zhu, Min, Sun, Yuyu, Xue, Huawei, Gu, Baorong, Yan, Xiaoling. 2023. NEK6 Promotes the Progression of Osteosarcoma Through Activating STAT3 Signaling Pathway by Down-Regulation of miR-26a-5p. In International journal of general medicine, 16, 2831-2848. doi:10.2147/IJGM.S413461. https://pubmed.ncbi.nlm.nih.gov/37426517/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen